Evidence map›Paper›PMID 35094328›Full record

ArticleMethods in molecular biology (Clifton, N.J.)

Node-Pore Sensing for Characterizing Cells and Extracellular Vesicles.

Thomas Carey, Brian Li, Lydia L Sohn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Thomas CareyThe UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Brian LiThe UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Lydia L SohnThe UC Berkeley-UC San Francisco Graduate Program in Bioengineering, University of California, Berkeley, Berkeley, CA, USA. sohn@berkeley.edu.

Funding

Mechanical Phenotyping of Random Periaerolar Fine Needle Aspiration-Collected Cells for Early Breast Cancer DetectionR01EB024989 · NIBIB · UNIVERSITY OF CALIFORNIA BERKELEY · PI Mark A LaBarge, Lydia L Sohn · 2017 to 2026
$4.8M
A Label-Free, Point-of-Care Platform to Diagnose Acute Promyelocytic LeukemiaR01CA190843 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI FEUSNER, JAMES, SOHN, LYDIA L · 2015 to 2019
$1.6M
Isolating Circulating Tumor CellsR21CA182375 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI SOHN, LYDIA L · 2015 to 2017
$605k
Node-Pore Sensing for Cellular ScreeningR21EB019181 · NIBIB · UNIVERSITY OF CALIFORNIA BERKELEY · PI LUSTIG, MICHAEL, SOHN, LYDIA L · 2015 to 2016
$409k
Rapid Label-Free Detection of Acute Promyelocytic LeukemiaR21CA156139 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI SOHN, LYDIA L · 2011 to 2012
$356k
NCI NIH HHS R01 CA190843NCI NIH HHS R21 CA156139NCI NIH HHS R21 CA182375NIBIB NIH HHS R01 EB024989NIBIB NIH HHS R21 EB019181
6 · The paper itself

Abstract

Node-Pore Sensing, NPS, is an extremely versatile and powerful technique for the analysis of cells and the detection of extracellular vesicles (EVs). NPS involves measuring the modulated current pulse caused by a cell transiting a microfluidic channel that has been segmented by a series of inserted nodes. As the current pulse reflects the number of nodes and segments of the channel, NPS can achieve exquisite sensitivity. Thus, when used as a Coulter counter, NPS can measure the sub-micron size increase of antibody-coated colloids to which EVs are specifically bound. By simply inserting between two nodes a "contraction" channel through which cells can squeeze, one can mechanically phenotype cells. We discuss the details of performing these two NPS applications.

Indexed as

Extracellular VesiclesColloidsMicrofluidicsColloidsCancer cellsExtracellular vesiclesMechano-phenotypingNode-pore sensingResistive-pulse sensingTumor marker

Identifiers

PMID35094328
PMCPMC10836821

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.