Evidence map›Paper›PMID 35094320›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Label-Free Morphological Phenotyping of In Vitro 3D Microtumors.

Zoe Moscato, Devina Jaiswal, Krishna Dixit, Cooper J Langanis, Kevin P Claffey, Kazunori Hoshino

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Zoe MoscatoDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.
Devina JaiswalDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.
Krishna DixitDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.
Cooper J LanganisDepartment of Biomedical Engineering, The State University of New York at Binghamton, Binghamton, NY, USA.
Kevin P ClaffeyDepartment of Cell Biology, University of Connecticut Health Center, Storrs, CT, USA.
Kazunori HoshinoDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT, USA. hoshino@engr.uconn.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

By combining novel micro-scale manipulation cantilevers with commercially available, widely used 3D light microscopy, we were able to develop a new method of 3D elastography specialized for the analysis of 3D microtumors. Existing mechanical characterization methods are available for the study of single cells, using forces in the range of sub pN to a few hundred nN, or of larger tissues, with forces greater than 1 mN. Our method supports the mechanical analysis of micro- to meso-scale 3D tissues, such as multicellular spheroids (200-300 μm diameter), by applying forces in the range of sub-hundred nN to sub-mN, while also maintaining a spatial resolution of elasticity measurement as small as 20-30 μm. We use a differential interference contrast (DIC)/confocal microscope to obtain a 4D (x, y, z, and indentation steps) image sequence, which is then analyzed using our custom 3D pattern-tracking MATLAB program. With this method, we have been able to show structural and spatial heterogeneity among single cells and surrounding regions in tumor spheroids, and between different cell types in tumor-fibroblast co-cultured spheroids. Our method has the potential to both bridge the gap between in vitro monolayer culture systems and in vivo animal studies and add a mechanical component to existing biological assays.

Indexed as

NeoplasmsSpheroids, CellularAnimalsCoculture TechniquesFibroblasts3D Elastography3D Light MicroscopyBreast CancerCo-CultureLabel-FreeMechanical AssayMicromanipulationMorphologyPhenotypingSpheroid

Identifiers

PMID35094320

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.