Evidence map›Paper›PMID 35094160›Full record

ArticleDevelopment genes and evolution2022

Mitochondrial gene expression analysis reveals aberrant transcription of cox3 in Gossypium barbadense CMS line H276A.

Aziz Khan, Xiangjun Kong, Xiaofang Liao, Jie Zheng, Jingyi You, Min Li, Reem M Hussain, Haneef Raza, Ruiyang Zhou

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Article in Development genes and evolution, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Cotton heterosis and hybrid cultivar development.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023
    Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Aziz Khan *Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China.
Xiangjun Kong *Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China.
Xiaofang LiaoKey Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China.
Jie Zheng *Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China.
Jingyi YouKey Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China.
Min LiKey Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China.
Reem M HussainState Key Laboratory of Agricultural Microbiology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Haneef RazaKey Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China.
Ruiyang ZhouKey Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China. ruiyangzhou@aliyun.com.
Guangxi University · CNCotton Research Institute · CNTishreen University · SY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cotton cytoplasmic male sterility (CMS) has been extensively studied; however, information regarding its molecular mechanisms has not yet been disclosed. Therefore, to explore the molecular mechanism of pollen abortion of cotton CMS line H276A, transcript profiles of 30 mitochondrial protein-encoding genes at tetrad stage were conducted with northern blot and a differential expression gene cox3 was identified. Quantitative reverse-transcribed PCR (qRT-PCR) analysis indicated that the expression level of cox3 in the CMS line H276A was 0.39-fold compared to its maintainer line H276B. In addition, the immunoblot analysis revealed that the amount of COX3 protein was decreased to 59.38% in CMS line H276A. The 5` and 3` terminals of the transcript of cox3 in two materials were determined simultaneously with circularized RNA reverse-transcribed PCR (CR-RT-PCR). The data indicated that seven 5` end of transcript of cox3 in H276A (-451/-464/-465/-467/-471/-472/-508 respect to ATG) were identified which were different from that of H276B (-411/-412). A total of 15 single nucleotide polymorphisms (SNPs) was detected by clone sequencing analysis of upstream of cox3. To our knowledge, we are the first to comprehensively analyze the transcripts of the mitochondrial genome in the cotton CMS line and to identify the 5` and 3` terminals of the transcript of cox3 in cotton. Our data will provide a framework for a better understanding of molecular mechanisms of CMS and mitochondrial gene expression in cotton.

Indexed as

GossypiumPlant InfertilityGene Expression ProfilingGene Expression Regulation, PlantGenes, MitochondrialCircularized RNA reverse-transcribed PCRCotton (Gossypium barbadense)Cytoplasmic male sterilityMitochondrial genes

Identifiers

PMID35094160
OpenAlexW4210525843

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.