Evidence map›Paper›PMID 35094149›Full record

ArticleCurrent genetics2022

Genome-wide analysis reveals a rhamnolipid-dependent modulation of flagellar genes in Pseudomonas aeruginosa PAO1.

Michele R Castro, Graciela M Dias, Tiago S Salles, Nubia M Cabral, Danielly C O Mariano, Hadassa L Oliveira, Eliana S F W Abdelhay, Renata Binato, Bianca C Neves

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In one paragraph

Article in Current genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. FTY720 Reduces the Biomass of Biofilms inAntibiotics (Basel, Switzerland) · 2024
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Michele R CastroDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
Graciela M DiasDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
Tiago S SallesDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
Nubia M CabralDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
Danielly C O MarianoDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
Hadassa L OliveiraDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
Eliana S F W AbdelhayNational Cancer Institute (INCA), Rio de Janeiro, Brazil.
Renata BinatoNational Cancer Institute (INCA), Rio de Janeiro, Brazil.
Bianca C NevesDepartment of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil. bcneves@iq.ufrj.br.ORCID http://orcid.org/0000-0002-6985-8828
Universidade Federal do Rio de Janeiro · BRInstituto Nacional de Câncer - INCA · BR

Funding

Petrobras 0050.0079375.12.9
6 · The paper itself

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen and an important model organism for the study of bacterial group behaviors, including cell motility and biofilm formation. Rhamnolipids play a pivotal role in biofilm formation and motility phenotypes in P. aeruginosa, possibly acting as wetting agents and mediating chemotactic stimuli. However, no biochemical mechanism or gene regulatory network has been investigated in regard to rhamnolipids' modulation of those group behaviors. Using DNA microarrays, we investigated the transcriptomic profiles in the stationary phase of growth of wild-type P. aeruginosa PAO1 and a rhlA-mutant strain, unable to produce rhamnolipids. A total of 134 genes were differentially expressed, comprising different functional categories, indicating a significant physiological difference between the rhamnolipid-producing and -non-producing strains. Interestingly, several flagellar genes are repressed in the mutant strain, which directly relates to the inability of the rhlA-minus strain to develop a swarming-motility phenotype. Supplementation with exogenous rhamnolipids has partially restored flagellar gene expression in the mutant strain. Our results show significant evidence that rhamnolipids, the major biosynthetic products of rhlABC pathway, seem to modulate gene expression in P. aeruginosa.

Indexed as

GlycolipidsPseudomonas aeruginosaBacterial ProteinsBacterial ProteinsGlycolipidsrhamnolipidFlagellaPseudomonas aeruginosaRhamnolipidsSwarming motilityTranscriptomic profile

Identifiers

PMID35094149
OpenAlexW4210572219

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.