Evidence map›Paper›PMID 35093118›Full record

ArticleJournal of neuroinflammation2022

TRPV4 contributes to ER stress and inflammation: implications for Parkinson's disease.

Na Liu, Liping Bai, Zhipeng Lu, Rou Gu, Dongdong Zhao, Fang Yan, Jie Bai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 1 pooled it
8.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Article
  4. Article
  5. Article
  6. Neuronal Calcium Signaling and Cytoskeletal Dynamics in Neurodegeneration.International journal of molecular sciences · 2026
    Review
  7. Article
  8. Article
  9. Article
  10. Article
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  12. Article
  13. Review
  14. Article
  15. Regulation of Collecting Lymphatic Vessel Contractile Function by TRPV4 Channels.Arteriosclerosis, thrombosis, and vascular biology · 2025
    Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Na LiuMedical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China.
Liping BaiMedical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China.
Zhipeng LuMedical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China.
Rou GuMedical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China.
Dongdong ZhaoMedical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China.
Fang YanMedical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China.
Jie BaiMedical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China. jiebai662001@126.com.ORCID http://orcid.org/0000-0003-1281-3159
Kunming University of Science and Technology · CN

Funding

National Natural Science Foundation of China U2002220The Yunling Scholar 1097821401
6 · The paper itself

Abstract

backgroundParkinson's disease (PD) is a progressive neurodegenerative disorder. Its molecular mechanism is still unclear, and pharmacological treatments are unsatisfactory. Transient receptor potential vanilloid 4 (TRPV4) is a nonselective Ca

methodsIntracerebral injection of an adeno-associated virus (AAV) into the substantia nigra (SN) of mice was used to knockdown or upregulate the expression of TRPV4 and intraperitoneal injection of MPTP. Rotarod and pole tests were used to evaluate the locomotor ability of mice. We used immunohistochemistry, Nissl staining and Western blot to detect the alterations in the number of tyrosine hydroxylase (TH)-positive neurons, Nissl-positive neurons, the levels of ER stress-associated molecules and proinflammatory cytokines in the SN.

resultsThe SN was transfected with AAV for 3 weeks and expressed the target protein with green fluorescence. Knockdown of TRPV4 via injection of a constructed AAV-TRPV4 shRNAi into the SN alleviated the movement deficits of PD mice. Upregulation of TRPV4 via injection of a constructed AAV-TRPV4 aggravated the above movement disorders. The expression of TRPV4 was upregulated in the SN of MPTP-treated mice. Injection of AAV-TRPV4 shRNAi into the SN rescued the number of TH-positive and Nissl-positive neurons in the SN decreased by MPTP, while injection of AAV-TRPV4 induced the opposite effect. Moreover, MPTP-decreased Sarco/endoplasmic reticulum Ca

conclusionsThese results suggest that TRPV4 mediates ER stress and inflammation pathways, contributing to the loss of dopamine (DA) neurons in the SN and movement deficits in PD mice. Moreover, this study provides a new perspective on molecular targets and gene therapies for the treatment of PD in the future.

Indexed as

MPTP PoisoningParkinson Disease1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridineAnimalsDisease Models, AnimalDopaminergic NeuronsInflammationMiceMice, Inbred C57BLSubstantia NigraTRPV Cation Channels1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridineTrpv4 protein, mouseTRPV Cation ChannelsER stressInflammationMPTPParkinson’s diseaseSNTRPV4

Identifiers

PMID35093118
PMCPMC8800324
OpenAlexW4210587649

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.