ArticleCurrent microbiology2022
CRISPR Interference (CRISPRi) Mediated Suppression of OmpR Gene in E. coli: An Alternative Approach to Inhibit Biofilm.
Article in Current microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- PotF Affects the Antibacterial Activity of Plantaricin BM-1 Against Escherichia coli K12 by Modulating Biofilm Formation and Cell Membrane Integrity.Probiotics and antimicrobial proteins · 2026Article
- Unravelling the Gut-Microbiome-Brain Axis: Implications for Infant Neurodevelopment and Future Therapeutics.Current microbiology · 2025Review
- Targeted inhibition of E. coli adhesion using antisense oligonucleotides: an approach to combat bacteria via CsrB targeting.World journal of microbiology & biotechnology · 2025Article
- Cell Sizes Matter for Industrial Bioproduction, a Case of Polyhydroxybutyrate.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Recent advances in gene-editing approaches for tackling antibiotic resistance threats: a review.Frontiers in cellular and infection microbiology · 2024Review
- Cinnamaldehyde nanoemulsion decorated with rhamnolipid for inhibition of methicillin-resistantFrontiers in microbiology · 2024Article
- Beyond antibiotics: CRISPR/Cas9 triumph over biofilm-associated antibiotic resistance infections.Frontiers in cellular and infection microbiology · 2024Review
- Transcriptome analysis of the biofilm formation mechanism ofFrontiers in microbiology · 2023Article
- CRISPRi-Mediated Gene Suppression Reveals Putative Reverse Transcriptase Gene PA0715 to Be a Global Regulator ofInfection and drug resistance · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilm plays an important role in the community and hospital-acquired infections. Especially E. coli biofilm that contributes towards the significant part of medical devices associated with microbial infections. OmpR/EnvZ, a two-component system, is one of the regulatory mechanisms involved in transcription regulation in response to environmental osmolarity changes. The main objective of this study was to elucidate the role of the OmpR/EnvZ two-component system in regulating the biofilm through curli and fimbriae (FimH gene), a contrary approach towards biofilm inhibition. In this study, the CRISPRi technique was used to suppress the expression of the OmpR gene. The RT-PCR assay was performed to quantify mRNA gene expression of curli and biofilm producing genes, and the data were further confirmed by different microscopic, spectroscopic and biofilm quantification assay (Crystal Violet). It is the first time we have shown downregulation of the OmpR gene in biofilm causing clinical isolates of E. coli, which further suppressed the FimH gene, leading to biofilm reduction. The crystal violet assay and microscopic studies also confirmed the biofilm reduction. We conclude that the OmpR gene of the OmpR/EnvZ two-component system could be one of the targets for biofilm mediated infection intervention. Our findings open new vistas to explore the pathways and targets to control biofilm mediated infections.
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