ArticleNature communications2022
Transcriptional changes in the mammary gland during lactation revealed by single cell sequencing of cells from human milk.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
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Who cites it
81 citing papers in PubMed, 102 citations in OpenAlex.
- Breast cancer prevention by prophylactic Lalba mRNA-LNP vaccination.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Integrated Single-Nucleus Multi-Omics Atlases Reveal Lineage Plasticity and Regulatory Networks of Luminal Epithelial Cells During Mammary Gland Lactation and Involution.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Preserving RNA quality when freezing of human milk samples must occur before extraction: Validation of methods utilized in a multi-center cohort study.Journal of mammary gland biology and neoplasia · 2026Article
- Dysregulated Efferocytosis and Immune Microenvironment in High Somatic Cell Count Dairy Cows.Journal of animal physiology and animal nutrition · 2026Article
- Comparative Transcriptomics Suggests that Breast Secretory Epithelium Reuses Gene Repertoires Conserved Across Vertebrates Beyond Mammals.Genome biology and evolution · 2026Article
- Human milk: insights on cell composition, organoids and emerging applications.Pediatric research · 2026Review
- Biosynthesis of human milk oligosaccharides (HMOs) in glycoengineered human cells.Glycobiology · 2026Article
- SOX9 and SEMA7A regulate cell plasticity in the postpartum mammary gland with implications for breast cancer.bioRxiv : the preprint server for biology · 2026Article
- ATF3 Modulates the Endoplasmic Reticulum Stress-Induced Impairment of Milk Synthesis in Bovine Mammary Epithelial Cells.International journal of molecular sciences · 2026Article
- Article
- Inferring gene-regulatory networks using epigenomic priors.iScience · 2026Article
- Single-cell spatial atlas of the aging human breast.Nature aging · 2026Article
- Article
- Methods for Preserving Human Milk Cellular and Milk Fat Globule RNA.Journal of mammary gland biology and neoplasia · 2026Article
- Divergent aging of nulliparous and parous mammary glands reveals IL33+ hybrid epithelial cells.Nature communications · 2026Article
- Does ovarian stimulation accelerate the progression of BI-RADS categories? a propensity score matching-based retrospective cohort study.Frontiers in endocrinology · 2026Article
- Single-cell transcriptomics of leukocyte-enriched human milk reveals a highly diverse and adapted myeloid compartment.PloS one · 2026Article
- Functional shifts in immune composition follow lactation stage in human milk.Frontiers in immunology · 2026Article
- Developing Mammary Gland Models for Biomedical Applications.Research (Washington, D.C.) · 2026Review
- Impact of lactation stage, maternal age, and parity on mammary epithelial cell populations in fresh milk.Scientific reports · 2025Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
Abstract
Under normal conditions, the most significant expansion and differentiation of the adult mammary gland occurs in response to systemic reproductive hormones during pregnancy and lactation to enable milk synthesis and secretion to sustain the offspring. However, human mammary tissue remodelling that takes place during pregnancy and lactation remains poorly understood due to the challenge of acquiring samples. We report here single-cell transcriptomic analysis of 110,744 viable breast cells isolated from human milk or non-lactating breast tissue, isolated from nine and seven donors, respectively. We found that human milk largely contains epithelial cells belonging to the luminal lineage and a repertoire of immune cells. Further transcriptomic analysis of the milk cells identified two distinct secretory cell types that shared similarities with luminal progenitors, but no populations comparable to hormone-responsive cells. Taken together, our data offers a reference map and a window into the cellular dynamics that occur during human lactation and may provide further insights on the interplay between pregnancy, lactation and breast cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.