Evidence map›Paper›PMID 35091220›Full record

ArticleWater research2022

Performance of NIST SRM® 2917 with 13 recreational water quality monitoring qPCR assays.

Jessica R Willis, Mano Sivaganesan, Richard A Haugland, Jason Kralj, Stephanie Servetas, Monique E Hunter, Scott A Jackson, Orin C Shanks

Erratum issuedOpen access · greenAbstract read
In one paragraph

Article in Water research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jessica R WillisU.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, USA.
Mano SivaganesanU.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, USA.
Richard A HauglandU.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, USA.
Jason KraljNational Institute of Standards and Technology, Biosystems and Biomaterials Division, Complex Microbial Systems Group, Gaithersburg, MD, USA.
Stephanie ServetasNational Institute of Standards and Technology, Biosystems and Biomaterials Division, Complex Microbial Systems Group, Gaithersburg, MD, USA.
Monique E HunterNational Institute of Standards and Technology, Biosystems and Biomaterials Division, Complex Microbial Systems Group, Gaithersburg, MD, USA.
Scott A JacksonNational Institute of Standards and Technology, Biosystems and Biomaterials Division, Complex Microbial Systems Group, Gaithersburg, MD, USA.
Orin C ShanksU.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, USA. Electronic address: shanks.orin@epa.gov.
Environmental Protection Agency · USNational Institute of Standards and Technology · US

Funding

Intramural EPA EPA999999
6 · The paper itself

Abstract

Fecal pollution remains a significant challenge for recreational water quality management worldwide. In response, there is a growing interest in the use of real-time quantitative PCR (qPCR) methods to achieve same-day notification of recreational water quality and associated public health risk as well as to characterize fecal pollution sources for targeted mitigation. However, successful widespread implementation of these technologies requires the development of and access to a high-quality standard control material. Here, we report a single laboratory qPCR performance assessment of the National Institute of Standards and Technology Standard Reference Material 2917 (NIST SRM® 2917), a linearized plasmid DNA construct that functions with 13 recreational water quality qPCR assays. Performance experiments indicate the generation of standard curves with amplification efficiencies ranging from 0.95 ± 0.006 to 0.99 ± 0.008 and coefficient of determination values (R

Indexed as

Water MicrobiologyWater QualityEnvironmental MonitoringFecesReal-Time Polymerase Chain ReactionWater PollutionMicrobial source trackingqPCRRapid fecal indicatorStandard control material

Identifiers

PMID35091220
PMCPMC10786215
OpenAlexW4207080611

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.