ArticleBMC research notes2022
Core clock regulators in dexamethasone-treated HEK 293T cells at 4 h intervals.
Article in BMC research notes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Article
- Temporal dynamics and functional annotation of transcriptome rhythmicity in HEK293T cells.PloS one · 2026Article
- The crucial role of circadian synchronization in bone marrow adipose tissue mesenchymal stem cells: insights into pathogenesis in Fanconi anemia and acute myeloid leukemia.Molecular biology reports · 2025Article
- Regulation of circadian gene activity in fibroblasts from ADHD patients through Rosiglitazone: a pilot study.Journal of neural transmission (Vienna, Austria : 1996) · 2025Article
- Reduced expression of the PER2 protein contributes to βActa biochimica et biophysica Sinica · 2025Article
- Endocrine regulation of circadian rhythms.Npj biological timing and sleep · 2025Review
- The Effects of Circadian Rhythm on Lead-Induced Toxicity in the DBC1.2 Olfactory Dark Basal Cell Line.Cells · 2025Article
- Refillable silicone pump with precise switching for timed therapeutic delivery.Frontiers in bioengineering and biotechnology · 2025Article
- Cysteine Oxidation Promotes Dimerization/Oligomerization of Circadian Protein Period 2.Biomolecules · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectiveThe study of the circadian clock and its mechanisms is easily facilitated through clock resetting in cell culture. Among the various established synchronizers of the circadian clock in cell culture (temperature, serum shock, glucocorticoids), the artificial glucocorticoid Dexamethasone (DEX) is the most widely used. DEX treatment as a protocol to reset the circadian clock in culture gives simple readout with minimal laboratory requirements. Even though there are many studies regarding clock resetting in culture using DEX, reference points or expression patterns of core clock genes and their protein products are scarce and sometimes contradict other works with similar methodology. We synchronise a cell line of human origin with DEX to be used for studies on circadian rhythms.
resultsWe treat HEK 293T cells with DEX and describe the patterns of mRNA and proteins of core clock regulators, while making a clear point on how CLOCK is less than an ideal molecule to help monitor rhythms in this cell line.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.