ArticleAmerican journal of physiology. Heart and circulatory physiology2022
Electronic cigarette exposure causes vascular endothelial dysfunction due to NADPH oxidase activation and eNOS uncoupling.
Article in American journal of physiology. Heart and circulatory physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 59 citations in OpenAlex.
- Early-onset atherosclerotic cardiovascular disease in young adults: Lifestyle, environmental, and psychosocial risk factors.International journal of cardiology. Cardiovascular risk and prevention · 2026Review
- Associations of exclusive e-cigarette use and dual use of e-cigarettes and combustible cigarettes with prevalent and incident hypertension: A systematic review and meta-analysis.American journal of preventive cardiology · 2026Review
- Nicotine-containing products and the cardiovascular system: mechanisms and global health implications.Nature reviews. Cardiology · 2026Review
- [Vaping-associated central retinal vein occlusion].Die Ophthalmologie · 2026Article
- E-cigarette Aerosol Containing Nicotine Increases Aortic Stiffness in Young Mice.Cardiovascular toxicology · 2026Article
- Oxidative stress mediates cardiac electrophysiological injury in inhalation exposure to flavored vaping products.Heart rhythm · 2026Article
- Coronary artery spasm: mechanisms, risk factors, and translational strategies for precision management.Frontiers in cardiovascular medicine · 2026Review
- HIIT versus MICT on Blood Pressure and Cardiac Biomarkers in L-NAME-induced Hypertensive Rats.International journal of exercise science · 2026Article
- Adipocytokines as Pathogenic Mediators and Therapeutic Targets in Metabolic Syndrome-Related Cardiocerebrovascular Events.Diabetes, metabolic syndrome and obesity : targets and therapy · 2026Review
- Cellular and Molecular Mechanisms of Vascular Endothelial Injury Induced by Cigarette Smoke and Cigarette Smoke Extract in Atherosclerosis.Journal of inflammation research · 2026Review
- E-cigarettes and cardiovascular health: a review of components, mechanisms, and clinical risk.American journal of cardiovascular disease · 2026Review
- Nicotine and the cardiovascular system: unmasking a global public health threat.European heart journal · 2025Article
- Nicotine influence on cerebrovascular and neurocognitive function with in utero electronic cigarette exposure.The Journal of physiology · 2025Article
- Exploring the Impact of E-cigarettes on Cardiovascular Health: Insights from Preclinical and Clinical Studies.Cardiovascular toxicology · 2025Review
- Chronic Pod-Mod E-Cigarette Aerosol Exposure Induces Aortic Dysfunction in Hypercholesterolemic Mice: Role of Oxidative Stress and Inflammation.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- E-Cigarette Smoke Exposure Elevates Renal MR Expression and Induces BP Elevation.Hypertension (Dallas, Tex. : 1979) · 2025Article
- Article
- Impact of E-Cigarettes on Fetal and Neonatal Lung Development: The Influence of Oxidative Stress and Inflammation.Antioxidants (Basel, Switzerland) · 2025Review
- Association between serum cotinine concentrations on red blood cell folate concentrations in pregnant women and the mediating role of lymphocytes: an NHANES Study.Archives of public health = Archives belges de sante publique · 2025Article
- Electronic cigarette vape decreases nitric oxide bioavailability in vascular smooth muscle cells via increased cytoglobin-mediated metabolism.Free radical biology & medicine · 2025Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
We recently reported a mouse model of chronic electronic cigarette (e-cig) exposure-induced cardiovascular pathology, where long-term exposure to e-cig vape (ECV) induces cardiac abnormalities, impairment of endothelial function, and systemic hypertension. Here, we delineate the underlying mechanisms of ECV-induced vascular endothelial dysfunction (VED), a central trigger of cardiovascular disease. C57/BL6 male mice were exposed to ECV generated from e-cig liquid containing 0, 6, or 24 mg/mL nicotine for 16 and 60 wk. Time-dependent elevation in blood pressure and systemic vascular resistance were observed, along with an impairment of acetylcholine-induced aortic relaxation in ECV-exposed mice, compared with air-exposed control. Decreased intravascular nitric oxide (NO) levels and increased superoxide generation with elevated 3-nitrotyrosine levels in the aorta of ECV-exposed mice were observed, indicating that ECV-induced superoxide reacts with NO to generate cytotoxic peroxynitrite. Exposure increased NADPH oxidase expression, supporting its role in ECV-induced superoxide generation. Downregulation of endothelial nitric oxide synthase (eNOS) expression and Akt-dependent eNOS phosphorylation occurred in the aorta of ECV-exposed mice, indicating that exposure inhibited de novo NO synthesis. Following ECV exposure, the critical NOS cofactor tetrahydrobiopterin was decreased, with a concomitant loss of its salvage enzyme, dihydrofolate reductase. NADPH oxidase and NOS inhibitors abrogated ECV-induced superoxide generation in the aorta of ECV-exposed mice. Together, our data demonstrate that ECV exposure activates NADPH oxidase and uncouples eNOS, causing a vicious cycle of superoxide generation and vascular oxidant stress that triggers VED and hypertension with predisposition to other cardiovascular disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.