Evidence map›Paper›PMID 35089577›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts.

Romana Köszagová, Eva Hrabárová, Lucia Achbergerová, Jozef Nahálka

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Romana KöszagováInstitute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Eva HrabárováInstitute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Lucia AchbergerováInstitute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Jozef NahálkaInstitute of Chemistry, Center for Glycomics, Department of Carbohydrate Enzymology, Laboratory of Synthetic Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic. nahalka@savba.sk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biocatalysis and biotransformations have a broad application in industrial synthetic chemistry. In addition to the whole cell catalysis, purified recombinant enzymes are successfully used for biocatalysis of specific chemical reactions. In this contribution, we report characterization, immobilization, and application of several model target enzymes (D-amino acid oxidase, sialic acid aldolase, maltodextrin phosphorylase, polyphosphate kinase, UDP-glucose pyrophosphorylase) physiologically aggregated within inclusion bodies retaining their biological activity as immobilized biocatalysts.

Indexed as

Enzymes, ImmobilizedInclusion BodiesBacteriaBiocatalysisBiotransformationEnzymes, ImmobilizedBiocatalysis/biotransformationEnzyme immobilizationInclusion bodiesRecombinant enzymes

Identifiers

PMID35089577

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.