ArticleMethods in molecular biology (Clifton, N.J.)2022
Solubilization and Refolding of Inclusion Body Proteins.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- A Nanobody of PEDV S1 Protein: Screening and Expression inBiomolecules · 2024Article
- Heterologous Expression of Difficult to Produce Proteins in Bacterial Systems.International journal of molecular sciences · 2024Article
- Heterologous expression of a deacetylase and its application in L-glufosinate preparation.Bioprocess and biosystems engineering · 2023Article
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2 authors.
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Abstract
Expression of heterologous proteins in E. coli often leads to the formation of protein aggregates known as inclusion bodies (IBs). Inclusion body aggregates pose a major hurdle in the recovery of bioactive proteins from E. coli. Usage of strong denaturing buffers for solubilization of bacterial IBs results in poor recovery of bioactive protein. Structure-function understanding of IBs in the last two decades have led to the development of several mild solubilization buffers, which improve the recovery of bioactive from IBs. Recently, combinatorial mild solubilization methods have paved the way for solubilization of wide range of inclusion bodies with appreciable refolding yield. Here, we describe a simple protocol for solubilization and refolding of an inclusion body protein with appreciable recovery.
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