Evidence map›Paper›PMID 35089566›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Recombinant Expression of Complex Proteins in Human Cell Lines.

Aline de Sousa Bomfim, Bruna Samhan Archangelo, Aline Sanches Pereira, Elisa Maria de Sousa Russo

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Aline de Sousa BomfimSchool of Pharmaceutical Sciences at Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Bruna Samhan ArchangeloSchool of Pharmaceutical Sciences at Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Aline Sanches PereiraSchool of Pharmaceutical Sciences at Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Elisa Maria de Sousa RussoSchool of Pharmaceutical Sciences at Ribeirão Preto, University of São Paulo, São Paulo, Brazil. elisa@fcfrp.usp.br.
Universidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Coagulation factors, as factor VII, VIII, and IX, are complex proteins which are very difficult to express. Blood coagulation factor IX is a vitamin K-dependent protein, and it has become a valuable biopharmaceutical in the treatment of hemophilia B. Here, we describe the techniques used to generate human cell lines producing human recombinant factor IX, as an example of complex protein, as well as in vitro characterization of this coagulation factor.To produce the FIX human adherent 293T SK-Hep-1 cells were used and stably modified by a lentiviral vector carrying the hFIX and the eGFP genes. The eGFP was employed as a reporter protein.

Indexed as

Factor IXHemophilia BCell LineGenetic VectorsHumansRecombinant ProteinsFactor IXRecombinant ProteinsComplex proteinsFactor IXGeneration of cell linesHuman cell linesProtein expressionRecombinant proteins

Identifiers

PMID35089566
OpenAlexW4210541814

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.