Evidence map›Paper›PMID 35089525›Full record

ArticleChinese journal of integrative medicine2023

Acute Developmental Toxicity of Panax notoginseng in Zebrafish Larvae.

Rong-Rong Wang, Ting Li, Lei Zhang, Zheng-Yan Hu, Li Zhou, Le-Tian Shan, Jia-Wei Huang, Lan Li

Abstract read
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In one paragraph

Article in Chinese journal of integrative medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. The application ofAnnals of translational medicine · 2025
    Review
  3. Review
  4. Article
  5. Review
  6. Nutrients · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Rong-Rong WangCollege of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Ting LiDepartment of Plastic and Aesthetic Center, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China.
Lei ZhangSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310058, China.
Zheng-Yan HuCenter for Disease Control and Prevention of Zhejiang Province, Hangzhou, 310051, China.
Li ZhouZhejiang Institute of Orthopedics and Traumatology, the First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, 310006, China.
Le-Tian ShanZhejiang Institute of Orthopedics and Traumatology, the First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, 310006, China.
Jia-Wei HuangCollege of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 310053, China.
Lan LiDepartment of Pediatrics, the First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, 310006, China. lilan99@zcmu.edu.cn.
Zhejiang Chinese Medical University · CNFirst Affiliated Hospital Zhejiang University · CNZhejiang Center for Disease Control and Prevention · CNZhejiang University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo evaluate toxicity of raw extract of Panax notoginseng (rPN) and decocted extract of PN (dPN) by a toxicological assay using zebrafish larvae, and explore the mechanism by RNA sequencing assay.

methodsZebrafish larvae was used to evaluate acute toxicity of PN in two forms: rPN and dPN. Three doses (0.5, 1.5, and 5.0 µ g/mL) of dPN were used to treat zebrafishes for evaluating the developmental toxicity. Behavior abnormalities, body weight, body length and number of vertebral roots were used as specific phenotypic endpoints. RNA sequencing (RNA-seq) assay was applied to clarify the mechanism of acute toxicity, followed by real time PCR (qPCR) for verification. High performance liquid chromatography analysis was performed to determine the chemoprofile of this herb.

resultsThe acute toxicity result showed that rPN exerted higher acute toxicity than dPN in inducing death of larval zebrafishes (P<0.01). After daily oral intake for 21 days, dPN at doses of 0.5, 1.5 and 5.0 µ g/mL decreased the body weight, body length, and vertebral number of larval zebrafishes, indicating developmental toxicity of dPN. No other adverse outcome was observed during the experimental period. RNA-seq data revealed 38 genes differentially expressed in dPN-treated zebrafishes, of which carboxypeptidase A1 (cpa1) and opioid growth factor receptor-like 2 (ogfrl2) were identified as functional genes in regulating body development of zebrafishes. qPCR data showed that dPN significantly down-regulated the mRNA expressions of cpa1 and ogfrl2 (both P<0.01), verifying cpa1 and ogfrl2 as target genes for dPN.

conclusionThis report uncovers the developmental toxicity of dPN, suggesting potential risk of its clinical application in children.

Indexed as

Panax notoginsengSaponinsAnimalsLarvaSequence Analysis, RNAZebrafishSaponinsdevelopmental toxicityPanax notoginsengRNA sequencingzebrafish larvae

Identifiers

PMID35089525
OpenAlexW4210593843

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.