ArticleeLife2022
Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Restoring RCAN1 dosage mitigates sleep and EEG abnormalities in a Down syndrome model.Neurobiology of disease · 2026Article
- Exposure to xylene early in life induces memory abnormalities of adult male mice.Experimental animals · 2026Article
- NanoPlex: A Universal Strategy to Multiplex Immunostainings for Fluorescence Microscopy Using Secondary Nanobodies.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Cross-Analysis of Single-Cell Transcriptomic Datasets Reveals Conserved Neurogenic Gene Signatures and New Insights Into Neural Stem Cell Aging.Aging cell · 2025Article
- Characterization of ROMK cellular heterogeneity along the mouse kidney thick ascending limb.Pflugers Archiv : European journal of physiology · 2025Article
- Astrocyte proliferation in the hippocampal dentate gyrus is suppressed across the lifespan of dystrophin-deficient mdx mice.Experimental physiology · 2025Article
- Multimodal transcriptomics reveal neurogenic aging trajectories and age-related regional inflammation in the dentate gyrus.Nature neuroscience · 2025Article
- Shift work schedules alter immune cell regulation and accelerate cognitive impairment during aging.Journal of neuroinflammation · 2025Article
- AI-based hardware and software tools in microscopy to boost research in immunology and virology.Frontiers in immunology · 2025Review
- Extent and activity of adult hippocampal neurogenesis.Frontiers in neuroscience · 2025Review
- Base editing ofeLife · 2024Article
- NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals.Nature communications · 2024Article
- TSWIFT, a novel method for iterative staining of embedded and mounted human brain sections.Scientific reports · 2024Article
- Mechanism and role of nuclear laminin B1 in cell senescence and malignant tumors.Cell death discovery · 2024Review
- Aging Modulates the Ability of Quiescent Radial Glia-Like Stem Cells in the Hippocampal Dentate Gyrus to be Recruited into Division by Pro-neurogenic Stimuli.Molecular neurobiology · 2024Article
- Fluctuation of lysosomal protein degradation in neural stem cells of the postnatal mouse brain.Development (Cambridge, England) · 2024Article
- Iterative Indirect Immunofluorescence Imaging (4i) on Adherent Cells and Tissue Sections.Bio-protocol · 2023Article
- Chronic in vivo imaging defines age-dependent alterations of neurogenesis in the mouse hippocampus.Nature aging · 2023Article
- Adult hippocampal neurogenesis in Alzheimer's disease: A roadmap to clinical relevance.Cell stem cell · 2023Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advancing age causes reduced hippocampal neurogenesis, associated with age-related cognitive decline. The spatial relationship of age-induced alterations in neural stem cells (NSCs) and surrounding cells within the hippocampal niche remains poorly understood due to limitations of antibody-based cellular phenotyping. We established iterative indirect immunofluorescence imaging (4i) in tissue sections, allowing for simultaneous detection of 18 proteins to characterize NSCs and surrounding cells in 2-, 6-, and 12-month-old mice. We show that reorganization of the dentate gyrus (DG) niche already occurs in middle-aged mice, paralleling the decline in neurogenesis. 4i-based tissue analysis of the DG identifies changes in cell-type contributions to the blood-brain barrier and microenvironments surrounding NSCs to play a pivotal role to preserve neurogenic permissiveness. The data provided represent a resource to characterize the principles causing alterations of stem cell-associated plasticity within the aging DG and provide a blueprint to analyze somatic stem cell niches across lifespan in complex tissues.
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Registered trials
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