Evidence map›Paper›PMID 35088927›Full record

ArticleBiotechnology journal2022

Dual-layered hydrogels allow complete genome recovery with nucleic acid cytometry.

Makiko N Hatori, Cyrus Modavi, Peng Xu, Daniel Weisgerber, Adam R Abate

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Makiko N HatoriDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, California, USA.
Cyrus ModaviDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, California, USA.
Peng XuDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, California, USA.
Daniel WeisgerberDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, California, USA.
Adam R AbateDepartment of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, California, USA.ORCID https://orcid.org/0000-0001-9614-4831
QB3 · US

Funding

Gigapixel digital PCR in Giant Unilamellar VesiclesR01EB019453 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R., CHIU, CHARLES YEN · 2014 to 2022
$3.9M
Microfluidic immunoprofiling for biomarker discovery in rheumatoid arthritisDP2AR068129 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R. · 2014 to 2014
$2.4M
Accurate and unbiased single cell sequencing with digital droplet MDAR01HG008978 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ABATE, ADAM R. · 2016 to 2018
$1.9M
NHGRI NIH HHS R01 HG008978NIAMS NIH HHS DP2 AR068129NIBIB NIH HHS R01 EB019453
6 · The paper itself

Abstract

Targeting specific cells for sequencing is important for applications in cancer, microbiology, and infectious disease. Nucleic acid cytometry (NAC) is a powerful approach for accomplishing this because it allows specific cells to be isolated based on sequence biomarkers that are otherwise impossible to detect. However, existing methods require specialized microfluidic devices, limiting adoption. Here, a modified workflow is described that uses particle-templated emulsification (PTE) and flow cytometry to conduct the essential steps of cell detection and sorting normally accomplished by microfluidics. Our microfluidic-free workflow allows facile isolation and sequencing of cells, viruses, and nucleic acids and thus provides a powerful enrichment approach for targeted sequencing applications.

Indexed as

Nucleic AcidsFlow CytometryHydrogelsLab-On-A-Chip DevicesMicrofluidicsHydrogelsNucleic AcidsenrichmentgenomicshydrogelsmicrofluidicsPCRsequencing

Identifiers

PMID35088927
PMCPMC9208836
OpenAlexW4210785414

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.