Evidence map›Paper›PMID 35088848›Full record

ArticleDevelopment (Cambridge, England)2022

Heterogeneous pdgfrb+ cells regulate coronary vessel development and revascularization during heart regeneration.

Subir Kapuria, Haipeng Bai, Juancarlos Fierros, Ying Huang, Feiyang Ma, Tyler Yoshida, Antonio Aguayo, Fatma Kok, Katie M Wiens, Joycelyn K Yip and 8 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Decoding Diabetes: Hub Genes as Pivotal Players in Cardiomyopathy and Kidney Disease.Endocrine, metabolic & immune disorders drug targets · 2026
    Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
  9. Craniofacial and cardiac defects inFrontiers in cell and developmental biology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 6 institutions in 3 countries.

Subir KapuriaDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0003-1307-5653
Haipeng BaiDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0003-3082-3078
Juancarlos FierrosDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0002-9225-4379
Ying HuangDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Feiyang MaDepartment of Molecular, Cell and Developmental Biology, College of Letters and Sciences, University of California Los Angeles, Los Angeles, CA 90095, USA.
Tyler YoshidaDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0003-2193-4817
Antonio AguayoDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Fatma KokDepartment of Molecular, Cell, and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.ORCID 0000-0002-2956-1253
Katie M WiensDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Joycelyn K YipLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA.
Megan L McCainLaboratory for Living Systems Engineering, Department of Biomedical Engineering, USC Viterbi School of Engineering, University of Southern California, Los Angeles, CA 90089, USA.ORCID 0000-0003-1908-6783
Matteo PellegriniDepartment of Molecular, Cell and Developmental Biology, College of Letters and Sciences, University of California Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0001-9355-9564
Mikiko NagashimaDepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.
Peter F HitchcockDepartment of Ophthalmology and Visual Sciences, University of Michigan, Ann Arbor, MI 48105, USA.ORCID 0000-0001-9454-2684
Naoki MochizukiDepartment of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Osaka, 564-8565, Japan.ORCID 0000-0002-3938-9602
Nathan D LawsonDepartment of Molecular, Cell, and Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA.ORCID 0000-0001-7788-9619
Michael M R HarrisonDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0003-1703-9879
Ching-Ling LienDepartment of Surgery, The Saban Research Institute and Heart Institute of Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0002-5100-9780
Children's Hospital of Los Angeles · USUniversity of Southern California · USUniversity of California, Los Angeles · USUniversity of Massachusetts Chan Medical School · USUniversity of Michigan · USNational Cerebral and Cardiovascular Center · JP

Funding

VIVARIUM MODULEP30EY007003 · NEI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI David Antonetti · 1987 to 2026
$17.6M
RETINAL DEVELOPMENT--DENDRITIC GROWTH SYNAPSER01EY007060 · NEI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HITCHCOCK, PETER F · 1987 to 2021
$7.0M
Embryonic origins of endothelial heterogeneityR35HL140017 · NHLBI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI LAWSON, NATHAN D · 2018 to 2023
$6.0M
Cardiac lymphatic vessels in heart development and regeneration.R01HL148706 · NHLBI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI LIEN, CHING-LING E · 2020 to 2023
$1.8M
Cxc12 chemokine signaling regulates synchronous development of coronary vessels and myocardiumR01HL130172 · NHLBI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI LIEN, CHING-LING E · 2017 to 2020
$1.7M
NEI NIH HHS P30 EY007003NEI NIH HHS R01 EY007060NHLBI NIH HHS R01 HL130172NHLBI NIH HHS R01 HL148706NHLBI NIH HHS R35 HL140017
6 · The paper itself

Abstract

Endothelial cells emerge from the atrioventricular canal to form coronary blood vessels in juvenile zebrafish hearts. We find that pdgfrb is first expressed in the epicardium around the atrioventricular canal and later becomes localized mainly in the mural cells. pdgfrb mutant fish show severe defects in mural cell recruitment and coronary vessel development. Single-cell RNA sequencing analyses identified pdgfrb+ cells as epicardium-derived cells (EPDCs) and mural cells. Mural cells associated with coronary arteries also express cxcl12b and smooth muscle cell markers. Interestingly, these mural cells remain associated with coronary arteries even in the absence of Pdgfrβ, although smooth muscle gene expression is downregulated. We find that pdgfrb expression dynamically changes in EPDCs of regenerating hearts. Differential gene expression analyses of pdgfrb+ EPDCs and mural cells suggest that they express genes that are important for regeneration after heart injuries. mdka was identified as a highly upregulated gene in pdgfrb+ cells during heart regeneration. However, pdgfrb but not mdka mutants show defects in heart regeneration after amputation. Our results demonstrate that heterogeneous pdgfrb+ cells are essential for coronary development and heart regeneration.

Indexed as

AnimalsCoronary VesselsEndothelial CellsGene Expression Regulation, DevelopmentalHeartMyocytes, Smooth MuscleOrganogenesisPericardiumReceptor, Platelet-Derived Growth Factor betaRegenerationZebrafishReceptor, Platelet-Derived Growth Factor betaCoronary vesselsEpicardiumMural cellspdgfrbZebrafish

Identifiers

PMID35088848
PMCPMC8918812
OpenAlexW4214564855

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.