Evidence map›Paper›PMID 35088376›Full record

ArticleMolecular biology reports2022

Exploring the genetic potential of Pakistani soybean cultivars through RNA-seq based transcriptome analysis.

Arslan Tariq, Zahra Jabeen, Sumaira Farrakh, Kiran Noreen, Waleed Arshad, Haroon Ahmed, Waseem Haider

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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3citing papers in PubMed
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3 · Its place in the literature

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3 citing papers in PubMed.

  1. Article
  2. Article
  3. Improvement of heat stress tolerance in soybean (Frontiers in plant science · 2022
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5 · Who and what money

Authors and funding

7 authors.

Arslan TariqDepartment of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan.
Zahra JabeenDepartment of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan.
Sumaira FarrakhDepartment of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan.
Kiran NoreenDepartment of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan.
Waleed ArshadDepartment of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan.
Haroon AhmedDepartment of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan.
Waseem HaiderDepartment of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan. waseem_haider@comsats.edu.pk.ORCID http://orcid.org/0000-0001-9200-6714

Funding

higher education commission, pakistan 7838/Balochistan/NRPU/R&D/HEC/2017
6 · The paper itself

Abstract

backgroundSoybean is largely grown and considered among the top oilseed crops. Three Pakistani cultivars, NARC-II (N), Swat-84 (S), and Rawal-I (R) were employed for RNA-Seq based transcriptome analysis to explore their genetic potential and performance in our local environment. METHODS AND

resultsWe grew the plants in glass house at same conditions and sampled leaves for RNA-Seq analysis in triplicate for each variety. We retrieved 2225 differentially expressed genes (DEGs) between S vs R, 2591 DEGs between S vs N, and 1221 DEGs between R vs N cultvars. These genes consist of transcription factors representing Basic Helix-loop Helix, myeloblastosis, ethylene response factors, and WRKY amino acid motif (WRKY) type major families that were up-regulated. KEGG pathway analysis revealed that MAPK, plant hormone signal transduction, and Phenylpropanoid biosynthesis pathways were the most dominant pathways involved in plant defense and growth. Comparative analysis showed that Swat-84 (S) cultivar had better gene expression among these varieties having higher number of DEGs, where mostly genes related to important phenotypic traits were up regulated.

conclusionsThis is a pilot study to investigate and functionally characterise the DEG involved in the stress response in the cultivars studied.

Indexed as

Gene Expression Regulation, PlantGlycine maxGene Expression ProfilingHumansPakistanPilot ProjectsRNA-SeqTranscriptomeDifferential gene expression (DGE)Pakistani soybeanRNA-SeqTranscription factors

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