Evidence map›Paper›PMID 35087920›Full record

ArticleBio-protocol2021

Preparation of Giant

Yihao Jiang, Vinay Idikuda, Baron Chanda

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Yihao JiangDepartment of Psychiatry of First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Vinay IdikudaDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Baron ChandaDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, Missouri, USA.
Washington University in St. Louis · USZhejiang University · CN

Funding

Biophysical mechanisms of gating and modulation in voltage-gated ion channel superfamilyR35NS116850 · NINDS · WASHINGTON UNIVERSITY · PI Baron Chanda · 2020 to 2026
$6.9M
Thermodynamics and Energetics of voltage-gated ion channelsR01NS081293 · NINDS · WASHINGTON UNIVERSITY · PI CHANDA, BARON · 2012 to 2019
$2.9M
Mechanisms of voltage- and ligand-activation in HCN channelsR01NS101723 · NINDS · WASHINGTON UNIVERSITY · PI CHANDA, BARON · 2017 to 2020
$2.0M
Synthetic design of an all-optical electrophysiology systemR21GM131662 · NIGMS · WASHINGTON UNIVERSITY · PI CHANDA, BARON, SCHOMAKER, JENNIFER MARIE · 2019 to 2020
$395k
NIGMS NIH HHS R21 GM131662NINDS NIH HHS R01 NS081293NINDS NIH HHS R01 NS101723NINDS NIH HHS R35 NS116850
6 · The paper itself

Abstract

Prokaryotic ion channels have been instrumental in furthering our understanding of many fundamental aspects of ion channels' structure and function. However, characterizing the biophysical properties of a prokaryotic ion channel in a native membrane system using patch-clamp electrophysiology is technically challenging. Patch-clamp is regarded as a gold standard technique to study ion channel properties in both native and heterologous expression systems. The presence of a cell wall and the small size of bacterial cells makes it impossible to directly patch clamp using microelectrodes. Here, we describe a method for the preparation of giant

Indexed as

Bacteria patch clampE. coli spheroplastElectrophysiologyIon channelsProkaryotes

Identifiers

PMID35087920
PMCPMC8720513
OpenAlexW4206417922

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.