ArticleNature immunology2022
Intravital three-photon microscopy allows visualization over the entire depth of mouse lymph nodes.
Article in Nature immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 67 citations in OpenAlex.
- Giant anomalous exciton-multiphoton nonlinearities in layered hybrid perovskites.Light, science & applications · 2026Article
- Spatial anchoring of lipid metabolism shapes immune fate in the tumor microenvironment.Lipids in health and disease · 2026Review
- 4D Time-lapse imaging of cell dynamics in human alveolar tissue by label-free higher harmonic generation microscopy.Journal of translational medicine · 2026Article
- Enhancing biomedical optical volumetric imaging via self-supervised orthogonal learning.Science advances · 2026Article
- Mechanisms of T cell activation: Integrating signaling pathways, experimental models, and therapeutic implications.EXCLI journal · 2026Review
- Liver sinusoid constraints make random search of CD8 T cells for Plasmodium parasites efficient.bioRxiv : the preprint server for biology · 2025Article
- Tissue type and tissue depth dependency of pulse broadening in three-photon microscopy: impact on third harmonic generation.Biomedical optics express · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Accessing the viscera: Technologies for interoception research.Current opinion in neurobiology · 2025Review
- Intravital imaging of peritubular microcirculation impairment in cisplatin-induced acute kidney injury.JCI insight · 2025Article
- Meningeal lymphatic drainage: novel insights into central nervous system disease.Signal transduction and targeted therapy · 2025Review
- Article
- Deep structural brain imaging via computational three-photon microscopy.Journal of biomedical optics · 2025Article
- Efficient, broadly tunable, hollow-fiber source of megawatt pulses for multiphoton microscopy.Biomedical optics express · 2025Article
- EZH2 inhibition enhances T cell immunotherapies by inducing lymphoma immunogenicity and improving T cell function.Cancer cell · 2025Article
- Importance of ultradian oscillations in neurogenesis during development and its implications for spinal cord regeneration.Frontiers in cell and developmental biology · 2025Review
- AI-based hardware and software tools in microscopy to boost research in immunology and virology.Frontiers in immunology · 2025Review
- Deep and dynamic metabolic and structural imaging in living tissues.Science advances · 2024Article
- Optimized intravital three-photon imaging of intact mouse tibia links plasma cell motility to functional states.iScience · 2024Article
- Profiling Dynamic Patterns of Single-Cell Motility.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Intravital confocal microscopy and two-photon microscopy are powerful tools to explore the dynamic behavior of immune cells in mouse lymph nodes (LNs), with penetration depth of ~100 and ~300 μm, respectively. Here, we used intravital three-photon microscopy to visualize the popliteal LN through its entire depth (600-900 μm). We determined the laser average power and pulse energy that caused measurable perturbation in lymphocyte migration. Long-wavelength three-photon imaging within permissible parameters was able to image the entire LN vasculature in vivo and measure CD8
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.