Evidence map›Paper›PMID 35087047›Full record

ArticleNature communications2022

Circularly polarised luminescence laser scanning confocal microscopy to study live cell chiral molecular interactions.

Patrycja Stachelek, Lewis MacKenzie, David Parker, Robert Pal

Abstract read
In one paragraph

Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed.

  1. Article
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  4. 3D-printed chiral luminous materials.Nature communications · 2026
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8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Patrycja Stachelek *Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK.ORCID 0000-0003-3002-3986
Lewis MacKenzie *Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK.ORCID 0000-0002-8151-0525
David ParkerDepartment of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK.ORCID 0000-0001-5281-5146
Robert PalDepartment of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK. robert.pal@durham.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB/S017615/1Biotechnology and Biological Sciences Research Council BB/T009268/1
6 · The paper itself

Abstract

The molecular machinery of life is founded on chiral building blocks, but no experimental technique is currently available to distinguish or monitor chiral systems in live cell bio-imaging studies. Luminescent chiral molecules encode a unique optical fingerprint within emitted circularly polarized light (CPL) carrying information about the molecular environment, conformation, and binding state. Here, we present a CPL Laser Scanning Confocal Microscope (CPL-LSCM) capable of simultaneous chiroptical contrast based live-cell imaging of endogenous and engineered CPL-active cellular probes. Further, we demonstrate that CPL-active probes can be activated using two-photon excitation, with complete CPL spectrum recovery. The combination of these two milestone results empowers the multidisciplinary imaging community, allowing the study of chiral interactions on a sub-cellular level in a new (chiral) light.

Indexed as

LuminescenceAnimalsLuminescent MeasurementsMiceMicroscopy, ConfocalMolecular ConformationNIH 3T3 CellsOptical ImagingOptical PhenomenaPolymersStereoisomerismPolymers

Identifiers

PMID35087047
PMCPMC8795401

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.