ArticleNature communications2022
Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed.
- Classification of pulmonary inflammation stages and assessment of multicomponent drug intervention based on spatiotemporal imaging variations utilizing RhB-conjugated poly-L-lysine nanoparticles.Journal of pharmaceutical analysis · 2026Article
- Simultaneous thermal and large field-of-view two-photon imaging enables optimization of signal-to-noise.Neurophotonics · 2026Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Compact module for video-rate image mosaics in two-photon microscopy.Optics express · 2025Article
- Review
- Three-dimensional random-access confocal microscopy with 3D remote focusing system.Communications engineering · 2024Article
- Article
- A Large Field-of-view, Single-cell-resolution Two- and Three-Photon Microscope for Deep Imaging.bioRxiv : the preprint server for biology · 2024Article
- Dimension-based quantification of aging-associated cerebral microvasculature determined by optical coherence tomography and two-photon microscopy.Journal of biophotonics · 2024Article
- Durable organic nonlinear optical membranes for thermotolerant lightings and in vivo bioimaging.Nature communications · 2023Article
- Multi-Mode Lanthanide-Doped Ratiometric Luminescent Nanothermometer for Near-Infrared Imaging within Biological Windows.Nanomaterials (Basel, Switzerland) · 2023Article
- Review
- Spectral inference reveals principal cone-integration rules of the zebrafish inner retina.Current biology : CB · 2021Article
- Colourfulness as a possible measure of object proximity in the larval zebrafish brain.Current biology : CB · 2021Article
- Zebrafish Retinal Ganglion Cells Asymmetrically Encode Spectral and Temporal Information across Visual Space.Current biology : CB · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Diffraction-limited two-photon microscopy permits minimally invasive optical monitoring of neuronal activity. However, most conventional two-photon microscopes impose significant constraints on the size of the imaging field-of-view and the specific shape of the effective excitation volume, thus limiting the scope of biological questions that can be addressed and the information obtainable. Here, employing a non-telecentric optical design, we present a low-cost, easily implemented and flexible solution to address these limitations, offering a several-fold expanded three-dimensional field of view. Moreover, rapid laser-focus control via an electrically tunable lens allows near-simultaneous imaging of remote regions separated in three dimensions and permits the bending of imaging planes to follow natural curvatures in biological structures. Crucially, our core design is readily implemented (and reversed) within a matter of hours, making it highly suitable as a base platform for further development. We demonstrate the application of our system for imaging neuronal activity in a variety of examples in zebrafish, mice and fruit flies.
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Registered trials
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