ArticleMicrobial biotechnology2022
Oligomerization engineering of the fluorinase enzyme leads to an active trimer that supports synthesis of fluorometabolites in vitro.
Article in Microbial biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 15 citations in OpenAlex.
- Bacterial Fluorinase FIA1 and Evolutionarily Related, Lysine-free StDUF62 Show Distinct Diastereoselectivity and Salt Sensitivity.ACS omega · 2025Article
- Synthetic metabolism forRSC chemical biology · 2022Article
- A Nonconventional Archaeal Fluorinase Identified by In Silico Mining for Enhanced Fluorine Biocatalysis.ACS catalysis · 2022Article
- Oligomerization engineering of the fluorinase enzyme leads to an active trimer that supports synthesis of fluorometabolites in vitro.Microbial biotechnology · 2022Article
- Identification of Two Novel Fluorinases FromFrontiers in bioengineering and biotechnology · 2022Article
- Biocatalytic synthesis of 2-fluoro-3-hydroxypropionic acid.Frontiers in bioengineering and biotechnology · 2022Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fluorinase enzyme represents the only biological mechanism capable of forming stable C-F bonds characterized in nature thus far, offering a biotechnological route to the biosynthesis of value-added organofluorines. The fluorinase is known to operate in a hexameric form, but the consequence(s) of the oligomerization status on the enzyme activity and its catalytic properties remain largely unknown. In this work, this aspect was explored by rationally engineering trimeric fluorinase variants that retained the same catalytic rate as the wild-type enzyme. These results ruled out hexamerization as a requisite for the fluorination activity. The Michaelis constant (K
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