Evidence map›Paper›PMID 35083711›Full record

ArticleActa parasitologica2022

Immunoreactivity of Brugia malayi Calreticulin and Its Domains with Sera of Different Categories of Bancroftian Filarial Patients.

Sunita Yadav, Smita Gupta, Mohd Saeed, Huma Mustafa, Jitendra Kumar Saxena, Puvvada Kalpana Murthy

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Article in Acta parasitologica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 61% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Sunita YadavDivision of Biochemistry, CSIR-Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow, 226031, India.
Smita GuptaDivision of Biochemistry, CSIR-Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow, 226031, India.
Mohd SaeedDivision of Biosciences, Integral University, Lucknow, 226026, India.
Huma MustafaDivision of Biosciences, Integral University, Lucknow, 226026, India.
Jitendra Kumar SaxenaDivision of Biochemistry, CSIR-Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow, 226031, India.
Puvvada Kalpana MurthyDivision of Parasitology, CSIR-Central Drug Research Institute, BS 10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow, 226031, India. drpkmurthy@gmail.com.ORCID http://orcid.org/0000-0002-3499-2621
Central Drug Research Institute · INIntegral University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeWe identified calreticulin in human filaria Brugia malayi (BmCRT) that shares 97% homology with Wuchereria bancrofti calreticulin (WbCRT), but only 56% with human calreticulin. We found that BmCRT binds C1q and prevents complement-mediated parasite death; immunization with BmCRT leads to parasite death in a rodent model of the infection. BmCRT could, therefore, be a potential vaccine candidate. In the present study, we determined the levels of BmCRT-reactive IgG and its isotype in bancroftian filarial subjects.

methodsRecombinant BmCRT (rBmCRT) was prepared, and the sera of endemic normal subjects (EN), microfilaraemics (Mf+) and chronic amicrofilaraemics (ChMf-) from a bancroftian filaria-endemic area and normal subjects from filaria-non-endemic area (NEN) were probed for IgG and its isotypes reacting with rBmCRT and its domains rN, rP and rC.

resultsrBmCRT and its rN domain-reactive IgG levels were high in EN and Mf+ groups; rC domain and rP domain showed moderate and very little reactivity, respectively. NEN sera were non-reactive. Moderate levels of rBmCRT-reactive IgG1, IgG3 and IgG4 in EN and Mf+ groups and low levels of IgG2 in Mf+ were found; IgG1 and IgG3 reactivity was found for rBmCRT and its rN domain only, while IgG4 reactivity was moderate for rN domain and low for rP and rC domains. While IgG reactivity was seen in all the endemic subjects, IgG isotype reactivity was found mostly in EN and Mf+ subjects.

conclusionsModerate levels of rBmCRT (and its rN domain)-reactive IgG and its isotypes are present in bancroftian subjects. Preponderance of IgG1 and IgG3 isotypes which bind and activate complement has relevance to vaccine potential of BmCRT.

Indexed as

Brugia malayiElephantiasis, FilarialVaccinesAnimalsAntibodies, HelminthAntigens, HelminthCalreticulinHumansImmunizationImmunoglobulin GAntibodies, HelminthAntigens, HelminthCalreticulinImmunoglobulin GVaccinesBmCRT and its domainsBrugia malayiCalreticulinFilarial patientsIgG isotypes

Identifiers

PMID35083711
OpenAlexW4210551764

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.