Evidence map›Paper›PMID 35083583›Full record

ArticleBiotechnology letters2022

Immobilization of Baeyer-Villiger monooxygenase from acetone grown Fusarium sp.

Michio Takagi, Kotchakorn T Sriwong, Ayaka Masuda, Nozomi Kawaguchi, Shusuke Fukui, Lan Huong Le Viet, Dai-Ichiro Kato, Takashi Kitayama, Mikio Fujii, Afifa Ayu Koesoema and 1 more

Abstract read
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In one paragraph

Article in Biotechnology letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Michio TakagiDepartment of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Kotchakorn T SriwongDepartment of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Ayaka MasudaDepartment of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Nozomi KawaguchiDepartment of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Shusuke FukuiDepartment of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Lan Huong Le VietDepartment of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Dai-Ichiro KatoGraduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima, 890-0065, Japan.
Takashi KitayamaFaculty of Agriculture, Kindai University, 3327-204, Nakamachi, Nara, Japan.
Mikio FujiiSchool of Pharmacy, International University of Health and Welfare, 2600-1 Kitakanemaru, Ohtawara, Tochigi, Japan.
Afifa Ayu KoesoemaResearch Institute for Interdisciplinary Science (RIIS), Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama-shi, 700-8530, Japan.
Tomoko MatsudaDepartment of Life Sciences and Technology, School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan. tmatsuda@bio.titech.ac.jp.ORCID http://orcid.org/0000-0002-8934-8198
Tokyo Institute of Technology · JPInternational University of Health and Welfare · JPKagoshima University · JPKindai University · JPOkayama University · JP

Funding

Foundation for Promotion of Material Science and Technology of Japan JPMJSP2106Japan Society for the Promotion of Science JP19K05560
6 · The paper itself

Abstract

objectiveA novel biocatalyst for Baeyer-Villiger oxidations is necessary for pharmaceutical and chemical industries, so this study aims to find a Baeyer-Villiger monooxygenase (BVMO) and to improve its stability by immobilization.

resultsAcetone, the simplest ketone, was selected as the only carbon source for the screening of microorganisms with a BVMO. A eukaryote, Fusarium sp. NBRC 109816, with a BVMO (FBVMO), was isolated from a soil sample. FBVMO was overexpressed in E. coli and successfully immobilized by the organic-inorganic nanocrystal formation method. The immobilization improved the thermostability of FBVMO. Substrate specificity investigation revealed that both free and immobilized FBVMO were found to show catalytic activities not only for Baeyer-Villiger oxidation of ketones to esters but also for oxidation of sulfides to sulfoxides. Furthermore, a preparative scale reaction using immobilized FBVMO was successfully conducted.

conclusionsFBVMO was discovered from an environmental sample, overexpressed in E. coli, and immobilized by the organic-inorganic nanocrystal formation method. The immobilization successfully improved its thermostability.

Indexed as

FusariumMixed Function OxygenasesAcetoneEscherichia coliKetonesOxidation-ReductionSubstrate SpecificityAcetoneKetonesMixed Function OxygenasesBaeyer–Villiger monooxygenaseFusarium sp. NBRC 109816ImmobilizationThermostability

Identifiers

PMID35083583
OpenAlexW4220653344

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.