Evidence map›Paper›PMID 35083578›Full record

ArticleMikrochimica acta2022

T7 Endonuclease I-mediated voltammetric detection of KRAS mutation coupled with horseradish peroxidase for signal amplification.

Pinky Chowdhury, Byung Seok Cha, Seokjoon Kim, Eun Sung Lee, Taehwi Yoon, Jisu Woo, Ki Soo Park

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Pinky ChowdhuryDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
Byung Seok ChaDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
Seokjoon KimDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
Eun Sung LeeDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
Taehwi YoonDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
Jisu WooDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea.
Ki Soo ParkDepartment of Biological Engineering, College of Engineering, Konkuk University, Seoul, Republic of Korea. akdong486@konkuk.ac.kr.ORCID http://orcid.org/0000-0002-0545-0970
Konkuk University · KR

Funding

Konkuk University Konkuk University Researcher Fund in 2021Korea Institute of Energy Technology Evaluation and Planning 20194010201900Korea Institute of Energy Technology Evaluation and Planning MOTIENational Research Foundation of Korea NRF-2020R1C1C1012275
6 · The paper itself

Abstract

Rapid and selective sensing of KRAS gene mutation which plays a crucial role in the development of colorectal, pancreatic, and lung cancers is of great significance in the early diagnosis of cancers. In the current study, we developed a simple electrochemical biosensor by differential pulse voltammetry technique for the specific detection of KRAS mutation that uses the mismatch-specific cleavage activity of T7-Endonuclease I (T7EI) coupled with horseradish peroxidase (HRP) to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) substrate in the presence of hydrogen peroxide (H

Indexed as

Cell Line, TumorChitosanColonic NeoplasmsDeoxyribonuclease IElectrochemical TechniquesElectrodesFemaleGene Expression Regulation, NeoplasticGoldHorseradish PeroxidaseHumansIonic LiquidsMetal NanoparticlesMutationNanotubes, CarbonProto-Oncogene Proteins p21(ras)ChitosanDeoxyribonuclease IGoldHorseradish PeroxidaseIonic LiquidsKRAS protein, humanNanotubes, CarbonProto-Oncogene Proteins p21(ras)Differential pulse voltammetryElectrochemical biosensorKRAS G12D mutationNanocompositeScreen-printed carbon electrodeT7-Endonuclease I (T7EI)

Identifiers

PMID35083578
OpenAlexW4210763334

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.