Evidence map›Paper›PMID 35082275›Full record

ArticleTranslational psychiatry2022

Dysregulated gene expression associated with inflammatory and translation pathways in activated monocytes from children with autism spectrum disorder.

Heather K Hughes, Megan E Rowland, Charity E Onore, Sally Rogers, Annie Vogel Ciernia, Paul Ashwood

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational psychiatry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
6.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.

  1. Pooled it
  2. Transcriptomic and Neuroimaging Decoding of Brain-Immune Crosstalk in Thyroid Eye Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
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  6. Review
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  10. Brain, behavior, & immunity - health · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Heather K HughesDepartment of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA.
Megan E RowlandDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.
Charity E OnoreDepartment of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA.
Sally RogersM.I.N.D. Institute, University of California, Davis, CA, USA.
Annie Vogel CierniaDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.
Paul AshwoodDepartment of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA. pashwood@ucdavis.edu.ORCID http://orcid.org/0000-0002-4255-1893
University of California, Davis · USVancouver Coastal Health · CAUniversity of California System · US

Funding

Immune regulation and autismR01MH118209 · NIMH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ASHWOOD, PAUL · 2019 to 2023
$2.0M
Immune regulation and gastrointestinal co-morbidity in autism spectrum disordersR01HD090214 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ASHWOOD, PAUL · 2018 to 2022
$1.9M
Maternal asthma and brain developmentR21MH116383 · NIMH · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ASHWOOD, PAUL · 2018 to 2019
$432k
NICHD NIH HHS R01 HD090214NIMH NIH HHS R01 MH118209NIMH NIH HHS R21 MH116383
6 · The paper itself

Abstract

Autism spectrum disorder (ASD) is a complex developmental disorder characterized by deficits in social interactions, communication, and stereotypical behaviors. Immune dysfunction is a common co-morbidity seen in ASD, with innate immune activation seen both in the brain and periphery. We previously identified significant differences in peripheral monocyte cytokine responses after stimulation with lipoteichoic acid (LTA) and lipopolysaccharide (LPS), which activate toll-like receptors (TLR)-2 and 4 respectively. However, an unbiased examination of monocyte gene expression in response to these stimulants had not yet been performed. To identify how TLR activation impacts gene expression in ASD monocytes, we isolated peripheral blood monocytes from 26 children diagnosed with autistic disorder (AD) or pervasive developmental disorder-not otherwise specified (PDDNOS) and 22 typically developing (TD) children and cultured them with LTA or LPS for 24 h, then performed RNA sequencing. Activation of both TLR2 and TLR4 induced expression of immune genes, with a subset that were differentially regulated in AD compared to TD samples. In response to LPS, monocytes from AD children showed a unique increase in KEGG pathways and GO terms that include key immune regulator genes. In contrast, monocytes from TD children showed a consistent decrease in expression of genes associated with translation in response to TLR stimulation. This decrease was not observed in AD or PDDNOS monocytes, suggesting a failure to properly downregulate a prolonged immune response in monocytes from children with ASD. As monocytes are involved in early orchestration of the immune response, our findings will help elucidate the mechanisms regulating immune dysfunction in ASD.

Indexed as

Autism Spectrum DisorderMonocytesChildCytokinesGene ExpressionHumansLeukocytes, MononuclearLipopolysaccharidesCytokinesLipopolysaccharides

Identifiers

PMID35082275
PMCPMC8791942
OpenAlexW4210489473

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.