ArticleThe Plant cell2022
Translation initiation landscape profiling reveals hidden open-reading frames required for the pathogenesis of tomato yellow leaf curl Thailand virus.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Characterization of the C4 protein encoded by parsley yellow leaf curl virus.Virology journal · 2026Article
- Pioneering plant virology research for a healthier future: a summary of the 2025 American Society for Virology (ASV) Plant Virology Satellite Symposium.Journal of virology · 2026Article
- The divergent V2 protein encoded by parsley yellow leaf curl virus is a suppressor of transcriptional and post-transcriptional gene silencing and a potential symptom determinant.BMC plant biology · 2026Article
- The Begomovirus Disease Tetrahedron: Weeds as the Missing Dimension in Virus Epidemiology.Viruses · 2026Review
- A pathogen-induced translational shift enhances plant disease resistance without obvious fitness costs.aBIOTECH · 2026Article
- A TIR-NLR gene from Arabidopsis Pla-1 confers resistance to geminivirus infection.The Plant journal : for cell and molecular biology · 2025Article
- TISCalling: leveraging machine learning to identify translational initiation sites in plants and viruses.Plant molecular biology · 2025Article
- Phylogeographic analysis ofThe Journal of general virology · 2024Article
- Optimization of ribosome profiling in plants including structural analysis of rRNA fragments.Plant methods · 2024Article
- Article
- Modeling alternative translation initiation sites in plants reveals evolutionarily conservedGenome research · 2024Article
- Reassortments in single-stranded DNA multipartite viruses: Confronting expectations based on molecular constraints with field observations.Virus evolution · 2024Review
- Hidden viral proteins: How powerful are they?PLoS pathogens · 2024Article
- Limited role of recombination in the global diversification of begomovirus DNA-B proteins.Virus research · 2023Article
- Article
- Exploiting Virus Infection to Protect Plants from Abiotic Stresses: Tomato Protection by a Begomovirus.Plants (Basel, Switzerland) · 2022Review
- Exciting times in plant biotic interactions.The Plant cell · 2022Article
- Virus secret revealed: ribosome profiling uncovers unannotated translation initiation sites and hidden open-reading frame in the TYLCTHV genome.The Plant cell · 2022Article
- Functional characterization of a new ORF βV1 encoded by radish leaf curl betasatellite.Frontiers in plant science · 2022Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant viruses with densely packed genomes employ noncanonical translational strategies to increase the coding capacity for viral function. However, the diverse translational strategies used make it challenging to define the full set of viral genes. Here, using tomato yellow leaf curl Thailand virus (TYLCTHV, genus Begomovirus) as a model system, we identified genes beyond the annotated gene sets by experimentally profiling in vivo translation initiation sites (TISs). We found that unanticipated AUG TISs were prevalent and determined that their usage involves alternative transcriptional and/or translational start sites and is associated with flanking mRNA sequences. Specifically, two downstream in-frame TISs were identified in the viral gene AV2. These TISs were conserved in the begomovirus lineage and led to the translation of different protein isoforms localized to cytoplasmic puncta and at the cell periphery, respectively. In addition, we found translational evidence of an unexplored gene, BV2. BV2 is conserved among TYLCTHV isolates and localizes to the endoplasmic reticulum and plasmodesmata. Mutations of AV2 isoforms and BV2 significantly attenuated disease symptoms in tomato (Solanum lycopersicum). In conclusion, our study pinpointing in vivo TISs untangles the coding complexity of a plant viral genome and, more importantly, illustrates the biological significance of the hidden open-reading frames encoding viral factors for pathogenicity.
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