ArticleeLife2022
Juxtacellular opto-tagging of hippocampal CA1 neurons in freely moving mice.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- HIPPIE: a generative model for electrophysiological analysis across species, technologies, and modalities.Nature communications · 2026Article
- Simultaneous path-integration recalibration in head direction and place cells.Current biology : CB · 2026Article
- Hippocampal structure, patterns of the calcium-binding proteins and neuron numbers in small echolocating bats.Frontiers in neuroanatomy · 2025Article
- Variations of neuronal properties in the region of locus coeruleus of mice.Brain research · 2024Article
- Acute optogenetic induction of the prodromal endophenotype of CA1 hyperactivity causes schizophrenia-related deficits in cognition and salience attribution.Schizophrenia (Heidelberg, Germany) · 2024Article
- Emerging technologies toward the integration of multiple functionalities on non-planar implantable neurophotonics probes.Neurophotonics · 2024Article
- Article
- Assessing cross-contamination in spike-sorted electrophysiology data.bioRxiv : the preprint server for biology · 2023Article
- High emotional reactivity is associated with activation of a molecularly distinct hippocampal-amygdala circuit modulated by the glucocorticoid receptor.Neurobiology of stress · 2023Article
- Opto-juxtacellular interrogation of neural circuits in freely moving mice.Nature protocols · 2023Review
- Optogenetic induction of hibernation-like state with modified human Opsin4 in mice.Cell reports methods · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neural circuits are made of a vast diversity of neuronal cell types. While immense progress has been made in classifying neurons based on morphological, molecular, and functional properties, understanding how this heterogeneity contributes to brain function during natural behavior has remained largely unresolved. In the present study, we combined the juxtacellular recording and labeling technique with optogenetics in freely moving mice. This allowed us to selectively target molecularly defined cell classes for in vivo single-cell recordings and morphological analysis. We validated this strategy in the CA1 region of the mouse hippocampus by restricting Channelrhodopsin expression to Calbindin-positive neurons. Directly versus indirectly light-activated neurons could be readily distinguished based on the latencies of light-evoked spikes, with juxtacellular labeling and post hoc histological analysis providing 'ground-truth' validation. Using these opto-juxtacellular procedures in freely moving mice, we found that Calbindin-positive CA1 pyramidal cells were weakly spatially modulated and conveyed less spatial information than Calbindin-negative neurons - pointing to pyramidal cell identity as a key determinant for neuronal recruitment into the hippocampal spatial map. Thus, our method complements current in vivo techniques by enabling optogenetic-assisted structure-function analysis of single neurons recorded during natural, unrestrained behavior.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.