ArticleChemMedChem2022
Desorption Electrospray Ionization Mass Spectrometry Assay for Label-Free Characterization of SULT2B1b Enzyme Kinetics.
Article in ChemMedChem, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Probing sulfotransferase binding and inhibition with synthetic PAPS analogs reveals the role of the 3'-phosphate and informs molecular tool design.RSC chemical biology · 2026Article
- Early-stage drug discovery in a new-generation ultrahigh-throughput mass spectrometry platform.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Ambient ionization mass spectrometry in brain cancer diagnosis.Journal of mass spectrometry and advances in the clinical lab · 2025Review
- High-Throughput Small-Scale Platform for Synthesis, Characterization, and Modeling of Per- and Polyfluoroalkyl Substances Analogs.Environmental science & technology letters · 2025Article
- Mechanisms of ionization and of chemical reactions in charged microdroplets.Chemical science · 2025Review
- Accelerating countermeasure candidate discovery for A-series chemical warfare agent exposure.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- High-Throughput Drug Derivatization and Bioassay by Desorption Electrospray Ionization Mass Spectrometry.ChemPlusChem · 2025Article
- Rapid Exploration of Chemical Space by High-Throughput Desorption Electrospray Ionization Mass Spectrometry.Journal of the American Chemical Society · 2024Article
- High-Throughput Assessment of Bile Salt Export Pump Inhibition Using RapidFire-MS and DESI-MS.ACS medicinal chemistry letters · 2024Article
- High-throughput label-free opioid receptor binding assays using an automated desorption electrospray ionization mass spectrometry platform.Chemical communications (Cambridge, England) · 2024Article
- Special Issue with Research Topics on "Recent Analysis and Applications of Mass Spectra on Biochemistry".International journal of molecular sciences · 2024Article
- Desorption Electrospray Ionization Mass Spectrometry: 20 Years.Accounts of chemical research · 2023Review
- Re-Imagining Drug Discovery using Mass Spectrometry.Israel journal of chemistry · 2023Article
- Applications of ambient ionization mass spectrometry in 2022: An annual review.Analytical science advances · 2023Review
- Recent Developments in Mass Spectrometry to Support Next-Generation Synthesis and Screening.ACS medicinal chemistry letters · 2023Review
- High-Throughput Diversification of Complex Bioactive Molecules by Accelerated Synthesis in Microdroplets.Angewandte Chemie (International ed. in English) · 2023Article
- Accelerated and Concerted Aza-Michael Addition and SuFEx Reaction in Microdroplets in Unitary and High-Throughput Formats.Angewandte Chemie (International ed. in English) · 2022Article
- High-throughput analysis of tissue microarrays using automated desorption electrospray ionization mass spectrometry.Scientific reports · 2022Article
- Spontaneous Water Radical Cation Oxidation at Double Bonds in Microdroplets.Frontiers in chemistry · 2022Article
- Complexity reduction and opportunities in the design, integration and intensification of biocatalytic processes for metabolite synthesis.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The sulfotransferase (SULT) 2B1b, which catalyzes the sulfonation of 3β-hydroxysteroids, has been identified as a potential target for prostate cancer treatment. However, a major limitation for SULT2B1b-targeted drug discovery is the lack of robust assays compatible with high-throughput screening and inconsistency in reported kinetic data. For this reason, we developed a novel label-free assay based on high-throughput (>1 Hz) desorption electrospray ionization mass spectrometry (DESI-MS) for the direct quantitation of the sulfoconjugated product (CV<10 %; <1 ng analyte). The performance of this DESI-based assay was compared against a new fluorometric coupled-enzyme method that we also developed. Both methodologies provided consistent kinetic data for the reaction of SULT2B1b with its major substrates, indicating the affinity trend pregnenolone>DHEA>cholesterol, for both the phospho-mimetic and wild-type SULT2B1b forms. The novel DESI-MS assay developed here is likely generalizable to other drug discovery efforts and is particularly promising for identification of SULT2B1b inhibitors with potential as prostate cancer therapeutics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.