Evidence map›Paper›PMID 35079104›Full record

ArticleCommunications biology2022

Haploidy in somatic cells is induced by mature oocytes in mice.

Yeonmi Lee, Aysha Trout, Nuria Marti-Gutierrez, Seoon Kang, Philip Xie, Aleksei Mikhalchenko, Bitnara Kim, Jiwan Choi, Seongjun So, Jongsuk Han and 14 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
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  5. Reproduction (Re)defined.Reproductive sciences (Thousand Oaks, Calif.) · 2025
    Article
  6. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors at 7 institutions in 3 countries.

Yeonmi Lee *Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
Aysha Trout *The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medicine, New York, NY, 10021, USA.
Nuria Marti-Gutierrez *Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Seoon KangDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.ORCID http://orcid.org/0000-0002-3947-4129
Philip XieThe Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medicine, New York, NY, 10021, USA.
Aleksei MikhalchenkoCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Bitnara KimDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
Jiwan ChoiDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
Seongjun SoDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
Jongsuk HanDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
Jing XuCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Amy KoskiCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.ORCID http://orcid.org/0000-0003-1918-528X
Hong MaCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Junchul David YoonCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.ORCID http://orcid.org/0000-0002-5481-9690
Crystal Van DykenCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.ORCID http://orcid.org/0000-0002-3293-9333
Hayley DarbyCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Dan LiangCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Ying LiCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Rebecca Tippner-HedgesCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Fuhua XuCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Paula AmatoCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Gianpiero D PalermoThe Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medicine, New York, NY, 10021, USA. gdpalerm@med.cornell.edu.ORCID http://orcid.org/0000-0001-8440-4560
Shoukhrat MitalipovCenter for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA. mitalipo@ohsu.edu.ORCID http://orcid.org/0000-0003-0937-6146
Eunju KangDepartment of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea. ekang@cha.ac.kr.ORCID http://orcid.org/0000-0002-7240-7868
Oregon Health & Science University · USCornell University · USUlsan College · KRCHA University · KRAdvanced Research Institute · USOregon National Primate Research Center · USStem Cell Institute · PA

Funding

Horizontal mtDNA ExchangeR01AG062459 · NIA · OREGON HEALTH & SCIENCE UNIVERSITY · PI MITALIPOV, SHOUKHRAT · 2019 to 2023
$2.8M
National Research Foundation of Korea (NRF) NRF-2018R1A2B3001244NIA NIH HHS R01 AG062459
6 · The paper itself

Abstract

Haploidy is naturally observed in gametes; however, attempts of experimentally inducing haploidy in somatic cells have not been successful. Here, we demonstrate that the replacement of meiotic spindles in mature metaphases II (MII) arrested oocytes with nuclei of somatic cells in the G0/G1 stage of cell cycle results in the formation of de novo spindles consisting of somatic homologous chromosomes comprising of single chromatids. Fertilization of such oocytes with sperm triggers the extrusion of one set of homologous chromosomes into the pseudo-polar body (PPB), resulting in a zygote with haploid somatic and sperm pronuclei (PN). Upon culture, 18% of somatic-sperm zygotes reach the blastocyst stage, and 16% of them possess heterozygous diploid genomes consisting of somatic haploid and sperm homologs across all chromosomes. We also generate embryonic stem cells and live offspring from somatic-sperm embryos. Our finding may offer an alternative strategy for generating oocytes carrying somatic genomes.

Indexed as

AnimalsChromosomesEmbryonic DevelopmentFemaleG1 Phase Cell Cycle CheckpointsG2 Phase Cell Cycle CheckpointsHaploidyMaleMiceMice, Inbred StrainsNuclear Transfer TechniquesOocytesSpindle Apparatus

Identifiers

PMID35079104
PMCPMC8789866
OpenAlexW4206905184

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.