ArticleThoracic cancer2022
CircRNA PRH1-PRR4 stimulates RAB3D to regulate the malignant progression of NSCLC by sponging miR-877-5p.
Article in Thoracic cancer, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- Parimifasor elicits broad-spectrum antitumor effects by suppressing the expression of oncogenic genes.Translational cancer research · 2026Article
- MicroRNAs in Breast Cancer Bone Metastasis Formation and Progression: An Overview on Recent Progress in This Research Field.Non-coding RNA · 2025Review
- MicroRNA-877-5p promotes osteoblast differentiation by targeting EIF4G2 expression.Journal of orthopaedic surgery and research · 2024Article
- Article
- Circular RNA in Non-small Cell Lung Carcinoma: Identification of Targets and New Treatment Modalities.Cancer genomics & proteomics · 2023Review
- Hsa_circ_0102899 promotes epithelial-mesenchymal transition in non-small cell lung cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2023Article
- Article
- Circ_0087378 intensifies the malignant behavior of non-small cell lung cancer cellsTranslational lung cancer research · 2023Article
- [miR-125b-5p inhibits proliferation and migration of osteosarcoma cells by negatively regulating RAB3D expression].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2023Article
- Circular RNA ATP2C1 (has_circ_0005797) sponges miR-432/miR-335 to promote breast cancer progression through regulating CCND1 expression.American journal of cancer research · 2023Article
- Multimodal AI for prediction of distant metastasis in carcinoma patients.Frontiers in bioinformatics · 2023Article
- Lymphatic metastasis-associated circRNA‒miRNA‒mRNA network for exploring the pathogenesis and therapeutic target of triple negative breast cancer based on whole-transcriptome sequencing analysis: an experimental verification study.Journal of translational medicine · 2022Article
- CircRNA PRH1-PRR4 stimulates RAB3D to regulate the malignant progression of NSCLC by sponging miR-877-5p.Thoracic cancer · 2022Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPrevious reports have confirmed the importance of circular RNA (circRNA) in the malignant progression of non-small-cell lung cancer (NSCLC). However, the role of circRNA PRH1-PRR4 readthrough (circPRH1-PRR4) in NSCLC progression was unclear. This study was designed to reveal the mechanism behind circPRH1-PRR4 regulating NSCLC progression.
methodsQuantitative real-time polymerase chain reaction and western blot were employed to detect the expression of circPRH1-PRR4, microRNA-877-5p (miR-877-5p), the member RAS oncogene family (RAB3D), and other indicated protein markers. The positive expression rate of RAB3D was detected by immunohistochemistry assay. Cell proliferation was investigated by cell colony formation and 5-ethynyl-2'-deoxyuridine assays. Flow cytometry was employed to quantify apoptotic cells. Wound-healing and transwell invasion assays were used to evaluate cell metastasis. The interaction among circPRH1-PRR4, miR-877-5p, and RAB3D was identified by dual-luciferase reporter assay. In vivo assay was implemented to demonstrate the effect of circPRH1-PRR4 on tumor formation.
resultsAs compared with controls, NSCLC tissues and cells displayed high expression of circPRH1-PRR4 and RAB3D, and low expression of miR-877-5p. Reduced expression of circPRH1-PRR4 resulted in inhibition of cell proliferation, migration, and invasion, but promotion of cell apoptosis in vitro. In support, circPRH1-PRR4 silencing inhibited tumor formation in vivo. Knockdown of miR-877-5p, a target miRNA of circPRH1-PRR4, relieved circPRH1-PRR4 absence-mediated action. Additionally, RAB3D was identified as a target mRNA of miR-877-5p. Importantly, circPRH1-PRR4 regulated RAB3D expression by miR-877-5p.
conclusionCircPRH1-PRR4 knockdown impeded NSCLC cell malignancy by the miR-877-5p/RAB3D pathway, providing a possible circRNA-targeted therapy for NSCLC.
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