Evidence map›Paper›PMID 35076587›Full record

ArticleNon-coding RNA2022

CLASH Analyst: A Web Server to Identify In Vivo RNA-RNA Interactions from CLASH Data.

Wei-Sheng Wu, Jordan S Brown, Pin-Hao Chen, Sheng-Cian Shiue, Dong-En Lee, Heng-Chi Lee

Open access · goldAbstract read
In one paragraph

Article in Non-coding RNA, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Analysis of crosslinking sites suggestsbioRxiv : the preprint server for biology · 2025
    Article
  3. Article
  4. Review
  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Wei-Sheng WuDepartment of Electrical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Jordan S BrownDepartment of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.ORCID 0000-0001-9880-4784
Pin-Hao ChenDepartment of Electrical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Sheng-Cian ShiueDepartment of Electrical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Dong-En LeeDepartment of Electrical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Heng-Chi LeeDepartment of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA.
National Cheng Kung University · TWUniversity of Chicago · US

Funding

PREDOCTORAL TRAINING IN GENETICS AND REGULATIONT32GM007197 · NIGMS · UNIVERSITY OF CHICAGO · PI ROTHMAN-DENES, LUCIA B. B · 1985 to 2020
$17.0M
piRNA-mediated genome surveillance of germline transcriptsR01GM132457 · NIGMS · UNIVERSITY OF CHICAGO · PI LEE, HENG-CHI · 2019 to 2023
$1.7M
Ministry of Science of Technology of Taiwan MOST-107-2221-E-006-225-MY3Ministry of Science of Technology of Taiwan MOST-108-2628-E-006-004-MY3NIGMS NIH HHS R01 GM132457NIGMS NIH HHS T32 GM007197NIH HHS GM132457NIH HHS T32 GM07197
6 · The paper itself

Abstract

Non-coding RNAs, such as miRNAs and piRNAs, play critical roles in gene regulation through base-pairing interactions with their target molecules. The recent development of the crosslinking, ligation, and sequencing of hybrids (CLASH) method has allowed scientists to map transcriptome-wide RNA-RNA interactions by identifying chimeric reads consisting of fragments from regulatory RNAs and their targets. However, analyzing CLASH data requires scientists to use advanced bioinformatics, and currently available tools are limited for users with little bioinformatic experience. In addition, many published CLASH studies do not show the full scope of RNA-RNA interactions that were captured, highlighting the importance of reanalyzing published data. Here, we present CLASH Analyst, a web server that can analyze raw CLASH data within a fully customizable and easy-to-use interface. CLASH Analyst accepts raw CLASH data as input and identifies the RNA chimeras containing the regulatory and target RNAs according to the user's interest. Detailed annotation of the captured RNA-RNA interactions is then presented for the user to visualize within the server or download for further analysis. We demonstrate that CLASH Analyst can identify miRNA- and piRNA-targeting sites reported from published CLASH data and should be applicable to analyze other RNA-RNA interactions. CLASH Analyst is freely available for academic use.

Indexed as

CLASHmiRNA targetsnon-coding RNApiRNA targetsRNA–RNA interactions

Identifiers

PMID35076587
PMCPMC8788457
OpenAlexW4206260599

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.