Evidence map›Paper›PMID 35076017›Full record

ArticleeLife2022

iMyoblasts for ex vivo and in vivo investigations of human myogenesis and disease modeling.

Dongsheng Guo, Katelyn Daman, Jennifer Jc Chen, Meng-Jiao Shi, Jing Yan, Zdenka Matijasevic, Amanda M Rickard, Monica H Bennett, Alex Kiselyov, Haowen Zhou and 6 more

Open access · goldAbstract read
In one paragraph

Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 28 citations in OpenAlex.

  1. SORT LNPs encapsulating Cas9 mRNA achieve efficient editing in skeletal muscle in a dystrophic mouse model.Molecular therapy : the journal of the American Society of Gene Therapy · 2026
    Article
  2. Review
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  4. PROTACs therapeutically target the polyglutamine androgen receptor in spinal and bulbar muscular atrophy models.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Dongsheng Guo *Wellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.ORCID 0000-0002-9702-7327
Katelyn Daman *Wellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.ORCID 0000-0003-1088-3903
Jennifer Jc ChenWellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.
Meng-Jiao ShiWellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.
Jing YanWellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.
Zdenka MatijasevicWellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.
Amanda M RickardGenea Biocells, La Jolla, United States.
Monica H BennettGenea Biocells, La Jolla, United States.
Alex KiselyovGenea Biocells, La Jolla, United States.
Haowen ZhouConrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.
Anne G BangConrad Prebys Center for Chemical Genomics, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, United States.
Kathryn R WagnerCenter for Genetic Muscle Disorders, Kennedy Krieger Institute, Baltimore, United States.
René MaehrProgram in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, United States.ORCID 0000-0002-9520-3382
Oliver D KingWellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.ORCID 0000-0002-9460-4491
Lawrence J HaywardWellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.ORCID 0000-0003-0579-0358
Charles P EmersonWellstone Muscular Dystrophy Program, Department of Neurology, University of Massachusetts Chan Medical School, Worcester, United States.ORCID 0000-0003-3744-9090
University of Massachusetts Chan Medical School · USSanford Burnham Prebys Medical Discovery Institute · USKennedy Krieger Institute · US

Funding

Myogenesis Studies for FSHD BiomarkersU54HD060848 · NICHD · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI EMERSON, CHARLES P. · 2008 to 2017
$16.1M
Training Core [Parent Title: NOVEL THERAPEUTICS FOR FSHD]P50HD060848 · NICHD · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI EMERSON, CHARLES P. · 2018 to 2023
$8.6M
CONTROL OF MUSCLE PROTEIN SYNTHESIS DURING MYOGENESISR37HD007796 · NICHD · UNIVERSITY OF PENNSYLVANIA · PI EMERSON, CHARLES P. · 2000 to 2010
$7.2M
NICHD NIH HHS P50 HD060848NICHD NIH HHS R37 HD007796NICHD NIH HHS U54 HD060848
6 · The paper itself

Abstract

Skeletal muscle myoblasts (iMyoblasts) were generated from human induced pluripotent stem cells (iPSCs) using an efficient and reliable transgene-free induction and stem cell selection protocol. Immunofluorescence, flow cytometry, qPCR, digital RNA expression profiling, and scRNA-Seq studies identify iMyoblasts as a

Indexed as

AnimalsCell DifferentiationCell LineCell LineageCells, CulturedDisease Models, AnimalHomeodomain ProteinsHumansInduced Pluripotent Stem CellsMiceMuscle DevelopmentMuscular Dystrophy, FacioscapulohumeralMyoblastsPAX3 Transcription FactorRecovery of FunctionRegenerationDux4 protein, mouseHomeodomain ProteinsPax3 protein, mousePAX3 Transcription Factordevelopmental biologyhumanhuman ipsc myogenesisiMyoblastsmousemuscle stem cellsregenerative medicinestem cellsxenograft

Identifiers

PMID35076017
PMCPMC8789283
OpenAlexW4207066841

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.