ArticleJournal of biomedical optics2022
High-speed mosaic imaging using scanner-synchronized stage position sampling.
Article in Journal of biomedical optics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The trial behind it
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Who cites it
9 citing papers in PubMed.
- Diagnostic accuracy of two-photon fluorescence microscopy in Mohs surgery of basal cell carcinomas.Journal of the American Academy of Dermatology · 2026Article
- Rapid evaluation of muscularis propria in transurethral resection of bladder tumour specimens using non-linear microscopy (NLM).Journal of clinical pathology · 2026Article
- High-throughput hyperspectral fluorescence imaging using a high-speed silicon photomultiplier array.Biomedical optics express · 2025Article
- High area and volumetric throughput parallel two-photon fluorescence microscopy using silicon photomultiplier arrays.Photonics research · 2025Article
- Vacuum-based specimen holder for rapid surgical specimen imaging.Biomedical optics express · 2025Article
- Suppression of Subpixel Jitter in Resonant Scanning Systems With Phase-locked Sampling.IEEE transactions on medical imaging · 2024Article
- Piston-based specimen holder for rapid surgical and biopsy specimen imaging.Biomedical optics express · 2024Article
- Rapid clearing and imaging of Mohs and melanoma surgical margins using a low-cost tissue processor.Biomedical optics express · 2024Article
- Optimal real-time resonant scanner linearization using filtered Hermite interpolation.Biomedical optics express · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
significanceTwo-photon and confocal microscopy can obtain high frame rates; however, mosaic imaging of large tissue specimens remains time-consuming and inefficient, with higher imaging rates leading to a larger fraction of time wasted translating between imaging locations. Strip scanning obtains faster mosaic imaging rates by translating a specimen at constant velocity through a line scanner at the expense of more complex stitching and geometric distortion due to the difficulty of translating at completely constant velocity.
aimWe aim to develop an approach to mosaic imaging that can obtain higher accuracy and faster imaging rates while reducing computational complexity. APPROACH: We introduce an approach based on scanner-synchronous position sampling that enables subwavelength accurate imaging of specimens moving at a nonuniform velocity, eliminating distortion.
resultsWe demonstrate that this approach increases mosaic imaging rates while reducing computational complexity, retaining high SNR, and retaining geometric accuracy.
conclusionsScanner synchronous strip scanning enables accurate, high-speed mosaic imaging of large specimens by reducing acquisition and processing time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.