ArticleThe New phytologist2022
Single-cell RNA-sequencing of Nicotiana attenuata corolla cells reveals the biosynthetic pathway of a floral scent.
Article in The New phytologist, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Exploring specialized metabolic pathways in medicinal plants with single-cell and spatial omics.Acta pharmaceutica Sinica. B · 2026Review
- Dynamic and cell-type specific transcriptional reprogramming underlies the floral transition in the maize shoot apical meristem.Genome biology · 2026Article
- Metabolomic and Single-Cell Transcriptomic Analyses Shed Light on Secondary Metabolite Profiling and Potential Developmental Dynamics of Glandular Trichomes in Artemisia argyi.Plant biotechnology journal · 2026Article
- Chemically guided single-cell transcriptomics reveals sulfotransferase-mediated scaffold remodeling in securinine biosynthesis.Nature communications · 2026Article
- Transcriptomic response analysis of rubber tree saplings to water-deficit stress at single-cell resolution.Communications biology · 2025Article
- Single-cell metabolome and RNA-seq multiplexing on single plant cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Evolution of petal patterning: blooming floral diversity at the microscale.The New phytologist · 2025Review
- Single-cell transcriptomic analyses reveal cellular and molecular patterns of rose petal responses to gray mold infection.Horticulture research · 2025Article
- Resolving floral development dynamics using genome and single-cell temporal transcriptome of Dendrobium devonianum.Plant biotechnology journal · 2025Article
- Applications and techniques of single-cell RNA sequencing across diverse species.Briefings in bioinformatics · 2025Review
- PhytoCluster: a generative deep learning model for clustering plant single-cell RNA-seq data.aBIOTECH · 2025Article
- Single-Cell Transcriptome Reveals the Cellular Response to PEG-Induced Stress in Wheat Leaves.Journal of agricultural and food chemistry · 2025Article
- Opportunities and challenges in the application of single-cell transcriptomics in plant tissue research.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Orthologous marker groups reveal broad cell identity conservation across plant single-cell transcriptomes.Nature communications · 2025Article
- The transcriptional integration of environmental cues with root cell type development.Plant physiology · 2024Review
- Single-Cell Transcriptomics Applied in Plants.Cells · 2024Review
- Single-cell RNA sequencing reveals a high-resolution cell atlas of petals inHorticulture research · 2024Article
- Quantitative Single-Cell Mass Spectrometry Provides a Highly Resolved Analysis of Natural Product Biosynthesis Partitioning in Plants.Journal of the American Chemical Society · 2024Article
- Genome assembly of Hibiscus sabdariffa L. provides insights into metabolisms of medicinal natural products.G3 (Bethesda, Md.) · 2024Article
- Opportunities and Challenges in Advancing Plant Research with Single-cell Omics.Genomics, proteomics & bioinformatics · 2024Review
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4 authors.
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Abstract
High-throughput single-cell RNA sequencing (scRNA-Seq) identifies distinct cell populations based on cell-to-cell heterogeneity in gene expression. By examining the distribution of the density of gene expression profiles, we can observe the metabolic features of each cell population. Here, we employ the scRNA-Seq technique to reveal the entire biosynthetic pathway of a flower volatile. The corolla of the wild tobacco Nicotiana attenuata emits a bouquet of scents that are composed mainly of benzylacetone (BA). Protoplasts from the N. attenuata corolla limbs and throat cups were isolated at three different time points, and the transcript levels of > 16 000 genes were analyzed in 3756 single cells. We performed unsupervised clustering analysis to determine which cell clusters were involved in BA biosynthesis. The biosynthetic pathway of BA was uncovered by analyzing gene co-expression in scRNA-Seq datasets and by silencing candidate genes in the corolla. In conclusion, the high-resolution spatiotemporal atlas of gene expression provided by scRNA-Seq reveals the molecular features underlying cell-type-specific metabolism in a plant.
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