ArticleNature genetics2022
A chromosomal loop anchor mediates bacterial genome organization.
Article in Nature genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Bacterial 3D genome architecture: organization, regulation, and synthetic biology applications.Genome biology · 2026Review
- An experimentally-informed polymer model reveals high resolution organization of genomic loci.Nature communications · 2026Article
- Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosis.Nucleic acids research · 2026Article
- Genome-wide DNA bridging by H-NS reshapes the stationary phase nucleoid and transcriptional landscape.bioRxiv : the preprint server for biology · 2025Article
- Nucleoid structure and dynamics influence natural chromosomal transformation in Bacillus subtilis: the role of EbfC.Nucleic acids research · 2025Article
- Principles of bacterial genome organization, a conformational point of view.Molecular microbiology · 2025Review
- Future Directions of the Prokaryotic Chromosome Field.Molecular microbiology · 2025Article
- Rok from B. subtilis: Bridging genome structure and transcription regulation.Molecular microbiology · 2025Review
- Hi-C/3C-seq Data Analysis for Prokaryotic Genomes with HiC-Pro.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Spatial chromosome organization and adaptation of the radiation-resistant extremophile Deinococcus radiodurans.The Journal of biological chemistry · 2025Article
- H-NS is a bacterial transposon capture protein.Nature communications · 2024Article
- Transcription factor shapes chromosomal conformation and regulates gene expression in bacterial adaptation.Nucleic acids research · 2024Article
- Implications of the three-dimensional chromatin organization for genome evolution in a fungal plant pathogen.Nature communications · 2024Article
- Chromosome structure modeling tools and their evaluation in bacteria.Briefings in bioinformatics · 2024Review
- Shedding Light on Bacterial Chromosome Structure: Exploring the Significance of 3C-Based Approaches.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Chromosome folding and prophage activation reveal specific genomic architecture for intestinal bacteria.Microbiome · 2023Article
- The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues.Nucleic acids research · 2022Article
- Cloning of MaizeInternational journal of molecular sciences · 2022Article
- HU Knew? Bacillus subtilis HBsu Is Required for DNA Replication Initiation.Journal of bacteriology · 2022Article
- Relationship between the Chromosome Structural Dynamics and Gene Expression-A Chicken and Egg Dilemma?Microorganisms · 2022Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleoprotein complexes play an integral role in genome organization of both eukaryotes and prokaryotes. Apart from their role in locally structuring and compacting DNA, several complexes are known to influence global organization by mediating long-range anchored chromosomal loop formation leading to spatial segregation of large sections of DNA. Such megabase-range interactions are ubiquitous in eukaryotes, but have not been demonstrated in prokaryotes. Here, using a genome-wide sedimentation-based approach, we found that a transcription factor, Rok, forms large nucleoprotein complexes in the bacterium Bacillus subtilis. Using chromosome conformation capture and live-imaging of DNA loci, we show that these complexes robustly interact with each other over large distances. Importantly, these Rok-dependent long-range interactions lead to anchored chromosomal loop formation, thereby spatially isolating large sections of DNA, as previously observed for insulator proteins in eukaryotes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.