Evidence map›Paper›PMID 35073603›Full record

ArticleFEBS open bio2022

SV40 T antigen helicase domain regions responsible for oligomerisation regulate Okazaki fragment synthesis initiation.

Nichodemus O Onwubiko, Felicia Scheffel, Ingrid Tessmer, Heinz Peter Nasheuer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Nichodemus O OnwubikoBiochemistry, School of Biological and Chemical Sciences, Biomedical Sciences Building, NUI Galway, Galway, Ireland.
Felicia ScheffelRudolf Virchow Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany.
Ingrid TessmerRudolf Virchow Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany.ORCID 0000-0002-8353-6315
Heinz Peter NasheuerBiochemistry, School of Biological and Chemical Sciences, Biomedical Sciences Building, NUI Galway, Galway, Ireland.ORCID 0000-0002-9218-9079
Ollscoil na Gaillimhe – University of Galway · IEUniversity of Würzburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The initiation of Okazaki fragment synthesis during cellular DNA replication is a crucial step for lagging strand synthesis, which is carried out by the primase function of DNA polymerase α-primase (Pol-prim). Since cellular replication protein A (RPA) prevents primase from starting RNA synthesis on single-stranded DNA (ssDNA), primase requires auxiliary factors, such as the simian virus 40 (SV40) T antigen (Tag), for the initiation reaction on RPA-bound ssDNA. Here, we investigated the ability of Tag variants and Tag protein complexes to bind to ssDNA and their resulting effects on the stimulation of Pol-prim on free and RPA-bound ssDNA. Atomic force microscopy imaging showed that while Tag

Indexed as

Antigens, Viral, TumorSimian virus 40DNAReplication Protein AAntigens, Viral, TumorDNAOkazaki fragmentsReplication Protein ADNA polymerase α-primase (Pol α)eukaryotic DNA replicationinitiation reactionOkazaki fragment synthesisreplication protein A (RPA)SV40 large T antigen

Identifiers

PMID35073603
PMCPMC8886539
OpenAlexW4206953401

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.