ArticlePloS one
Peripheral mitochondrial DNA, telomere length and DNA methylation as predictors of live birth in in vitro fertilization cycles.
Article in PloS one. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- LINE-1 Methylation sustains telomere length in pregnant women: effects on pregnancy failure.Clinical epigenetics · 2025Article
- Epigenetic age and fertility timeline: testing an epigenetic clock to forecast in vitro fertilization success rate.Reproductive biology and endocrinology : RB&E · 2025Observational
- The interplay between mitochondrial DNA genotypes, female infertility, ovarian response, and mutagenesis in oocytes.Human reproduction open · 2025Article
- Epigenetic aging and fecundability: the Norwegian Mother, Father and Child Cohort Study.Human reproduction (Oxford, England) · 2024Article
- Epigenetic age acceleration in follicular fluid and markers of ovarian response among women undergoing IVF.Human reproduction (Oxford, England) · 2024Article
- Telomeres and SIRT1 as Biomarkers of Gamete Oxidative Stress, Fertility, and Potential IVF Outcome.International journal of molecular sciences · 2024Review
- Evaluation of telomere length and telomerase activity on predicting in vitro fertilization treatment outcomes.Journal of assisted reproduction and genetics · 2024Review
- Epigenetic role of LINE-1 methylation and key genes in pregnancy maintenance.Scientific reports · 2024Article
- The role of epigenetics in women's reproductive health: the impact of environmental factors.Frontiers in endocrinology · 2024Review
- Article
- Potential effects of assisted reproductive technology on telomere length and telomerase activity in human oocytes and early embryos.Journal of ovarian research · 2023Review
- Epigenetic clocks and female fertility timeline: A new approach to an old issue?Frontiers in cell and developmental biology · 2023Review
- The Interplay between Telomeres, Mitochondria, and Chronic Stress Exposure in the Aging Egg.Cells · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo evaluate whether telomere length (TL), mitochondrial-DNA (mt-DNA) or epigenetic age estimators based on DNA methylation (DNAm) pattern could be considered reliable predictors of in-vitro-fertilization (IVF) success in terms of live birth rate.
designProspective cohort study.
settingInfertility Unit of the Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico. PATIENTS: 181 women aged 37-39 years who underwent IVF at a single-centre between January 2017 and December 2018.
interventionsOn the day of recruitment, blood samples were collected, and genomic DNA was isolated from white blood cells. TL, mt-DNA and DNAm assessment was performed using quantitative real-time polymerase chain reaction (qPCR). Biological age (DNAm age) was computed as the algorithm based on methylation pattern of five genes. Epigenetic age acceleration was estimated from the residuals of the linear model of epigenetic age regressed on chronological age. Long Interspersed Nuclear Elements (LINE)-1 methylation pattern was used as a surrogate for global DNA methylation.
main outcome measuresThis study investigated whether peripheral TL, mt-DNA and DNAm could predict live birth in IVF cycles.
resultsTL, mt-DNA and LINE-1 methylation were not associated with IVF success. Conversely, DNAm age resulted significantly lower in women who had a live birth compared to women who did not (36.1 ± 4.2 and 37.3 ± 3.3 years, respectively, p = 0.04). For DNAm age, odds ratio (OR) for live birth per year of age was 0.90 (95%CI: 0.82-0.99, p = 0.036) after adjusting for FSH and antral follicle count (AFC) and 0.90 (95%CI: 0.82-0.99, p = 0.028) after adjusting also for number of oocytes retrieved. A significant association also emerged for epigenetic age acceleration after adjustments (OR = 0.91, 95%CI: 0.83-1.00, p = 0.048).
conclusionDNAm age is associated with IVF success but the magnitude of this association is insufficient to claim a clinical use. However, our findings are promising and warrant further investigation. Assessment of biological age using different epigenetic clocks or focusing on different tissues may reveal new predictors of IVF success.
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