ArticleeLife2022
Multiphoton imaging of neural structure and activity in
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Exploring dose-dependent outcomes of photobiomodulation with two infrared wavelengths applied to injured Drosophila retinae.PloS one · 2026Article
- The Neuropeptide Neuroparsin-A Regulates the Establishment of Dominance Hierarchy in Bumblebees.International journal of molecular sciences · 2025Article
- Integrative functional optical imaging approaches: Optics, microfluidics, and machine learning for neuroscience in organoids and small-animal models.Biophysical journal · 2025Review
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Three-photon in vivo imaging of neurons and glia in the medial prefrontal cortex with sub-cellular resolution.Communications biology · 2025Article
- Behavioral Assays for Optogenetic Manipulation of Neural Circuits in Drosophila melanogaster.Journal of visualized experiments : JoVE · 2025Article
- Efficient, broadly tunable, hollow-fiber source of megawatt pulses for multiphoton microscopy.Biomedical optics express · 2025Article
- Review
- Measurement of third order coherence by in situ autocorrelation for determining three-photon cross-sections.Biomedical optics express · 2024Article
- Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cordbioRxiv : the preprint server for biology · 2024Article
- From connectome to effectome: learning the causal interaction map of the fly brain.bioRxiv : the preprint server for biology · 2024Article
- Visual feedback neurons fine-tune Drosophila male courtship via GABA-mediated inhibition.Current biology : CB · 2023Article
- Measurement of three-photon excitation cross-sections of fluorescein from 1154 nm to 1500 nm.Biomedical optics express · 2023Article
- Article
- Tilt in Place Microscopy: a Simple, Low-Cost Solution to Image Neural Responses to Body Rotations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- GABA-mediated inhibition in visual feedback neurons fine-tunesbioRxiv : the preprint server for biology · 2023Article
- Three-photon excited fluorescence imaging in neuroscience: From principles to applications.Frontiers in neuroscience · 2023Review
- Fast, efficient, and accurate neuro-imaging denoising via supervised deep learning.Nature communications · 2022Article
- Imaging whole-brain activity to understand behavior.Nature reviews. Physics · 2022Article
- Dual-wavelength multimodal multiphoton microscope with SMA-based depth scanning.Biomedical optics express · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We developed a multiphoton imaging method to capture neural structure and activity in behaving flies through the intact cuticle. Our measurements showed that the fly head cuticle has surprisingly high transmission at wavelengths >900nm, and the difficulty of through-cuticle imaging is due to the air sacs and/or fat tissue underneath the head cuticle. By compressing or removing the air sacs, we performed multiphoton imaging of the fly brain through the intact cuticle. Our anatomical and functional imaging results show that 2- and 3-photon imaging are comparable in superficial regions such as the mushroom body, but 3-photon imaging is superior in deeper regions such as the central complex and beyond. We further demonstrated 2-photon through-cuticle functional imaging of odor-evoked calcium responses from the mushroom body γ-lobes in behaving flies short term and long term. The through-cuticle imaging method developed here extends the time limits of in vivo imaging in flies and opens new ways to capture neural structure and activity from the fly brain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.