ArticleApplied microbiology and biotechnology2022
Rapid Russula senecis identification assays using loop-mediated isothermal amplification based on real-time fluorescence and visualization.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Review
- Development of Loop-Mediated Isothermal Amplification (LAMP) Methods for Rapid Identification of NephrotoxicMycobiology · 2026Article
- A systematic review of sensors to combat crime and routes to further sensor development.Frontiers in chemistry · 2025Review
- The method based on ATR-FTIR spectroscopy combined with feature variable selection for the boletus species and origins identification.Food science & nutrition · 2024Article
- Rapid analysis technologies with chemometrics for food authenticity field: A review.Current research in food science · 2024Review
- Article
- Development of a loop-mediated isothermal amplification assay for the rapid detection ofFrontiers in microbiology · 2022Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Russula senecis, a common poisonous mushroom, is widely distributed in China. Mushroom poisoning is becoming a major threat to human health and its rate is increasing worldwide. For the first time, we developed a set of loop-mediated isothermal amplification (LAMP) assays based on a real-time fluorescence and a visualization method to detect R. senecis, and the visual LAMP reaction system was optimized to further shorten the reaction time. Both real-time LAMP and visual LAMP could detect as low as 3.2 pg of genomic DNA. In addition, fried and digested mushrooms were used to validate the proposed LAMP method, and mushroom mixtures with as low as 1% of the target species could be successfully detected, indicating that the LAMP assays established in this study had good applicability and could be used for clinical sample detection and forensic identification. Furthermore, the LAMP assays were proven to be comparable to the real-time PCR method. KEY POINTS: • A set of loop-mediated isothermal amplification (LAMP) assays based on real-time fluorescence and visualization to detect Russula senecis was developed. • Both real-time LAMP and visual LAMP can be used to detect genomic DNA at concentrations as low as 3.2 pg. • By simulating mushroom processing and digestion in gastric juice, LAMP assays were proved to have good applicability and could be used for clinical diagnosis and forensic analysis.
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