Evidence map›Paper›PMID 35071046›Full record

ArticleFrontiers in cellular and infection microbiology2021

Crystal Violet Staining Alone Is Not Adequate to Assess Synergism or Antagonism in Multi-Species Biofilms of Bacteria Associated With Bacterial Vaginosis.

Joana Castro, Ângela Lima, Lúcia G V Sousa, Aliona S Rosca, Christina A Muzny, Nuno Cerca

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 2 pooled it
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 45 citations in OpenAlex.

  1. A systematic review of the use of bacteriophages for in vitro biofilm control.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023
    Pooled it
  2. Understanding bacterial biofilms: From definition to treatment strategies.Frontiers in cellular and infection microbiology · 2023
    Pooled it
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  10. Disruption ofMicrobiology (Reading, England) · 2025
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  18. Frontiers in cellular and infection microbiology · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Joana CastroCentre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), University of Minho, Braga, Portugal.
Ângela LimaCentre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), University of Minho, Braga, Portugal.
Lúcia G V SousaCentre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), University of Minho, Braga, Portugal.
Aliona S RoscaCentre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), University of Minho, Braga, Portugal.
Christina A MuznyDivision of Infectious Diseases, University of Alabama at Birmingham, Birmingham, AL, United States.
Nuno CercaCentre of Biological Engineering (CEB), Laboratory of Research in Biofilms Rosário Oliveira (LIBRO), University of Minho, Braga, Portugal.
University of Minho · PTUniversity of Alabama at Birmingham · US

Funding

Microbial Interactions between Gardnerella, Prevotella, and Atopobium Prior to Incident Bacterial VaginosisR01AI146065 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MUZNY, CHRISTINA A · 2020 to 2024
$3.2M
NIAID NIH HHS R01 AI146065
6 · The paper itself

Abstract

Bacterial Vaginosis (BV) involves the presence of a multi-species biofilm adhered to vaginal epithelial cells, but its in-depth study has been limited due to the complexity of the bacterial community, which makes the design of

Indexed as

Vaginosis, BacterialBacteriaBiofilmsFemaleGentian VioletHumansStaining and LabelingVaginaGentian Violetanaerobic bacteriabacterial vaginosisbiofilm quantificationcrystal violet stainingmicrotiter plates

Identifiers

PMID35071046
PMCPMC8766793
OpenAlexW4206402562

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.