Evidence map›Paper›PMID 35070455›Full record

ArticleInternational journal of hepatology2022

Disproportionate Distribution of HBV Genotypes A and D and the Recombinant Genotype D/E in the High and Low HBV Endemic Regions of Uganda: A Wake-Up Call for Regional Specific HBV Management.

Hussein Mukasa Kafeero, Dorothy Ndagire, Ponsiano Ocama, Charles Drago Kato, Eddie Wampande, Henry Kajumbula, David Kateete, Abdul Walusansa, Ali Kudamba, Kigozi Edgar and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of hepatology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Hussein Mukasa KafeeroDepartment of Microbiology, Makerere University, College of Health Sciences, P. O. Box 7062, Kampala, Uganda.ORCID https://orcid.org/0000-0002-9418-2831
Dorothy NdagireDepartment of Plant Sciences, Microbiology and Biotechnology, College of Natural Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Ponsiano OcamaDepartment of Medicine, College of Health Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Charles Drago KatoDepartment of Biomolecular Resources & Biolab Sciences (BBS) College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O. Box 7062, Kampala, Uganda.
Eddie WampandeDepartment of Biomolecular Resources & Biolab Sciences (BBS) College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O. Box 7062, Kampala, Uganda.
Henry KajumbulaDepartment of Microbiology, Makerere University, College of Health Sciences, P. O. Box 7062, Kampala, Uganda.
David KateeteDepartment of Molecular Biology and Immunology, College of Health Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Abdul WalusansaDepartment of Medical Microbiology, Habib Medical School, Faculty of Health Sciences, Islamic University in Uganda, P.O. Box 7689, Kampala, Uganda.
Ali KudambaDepartment of Physiology, Habib Medical School, Faculty of Health Sciences, Islamic University in Uganda, P.O. Box 7689, Kampala, Uganda.
Kigozi EdgarDepartment of Molecular Biology and Immunology, College of Health Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Fred Ashaba KatabaziDepartment of Molecular Biology and Immunology, College of Health Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Maria Magdalene NamagandaDepartment of Molecular Biology and Immunology, College of Health Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Jamilu E SsenkuDepartment of Plant Sciences, Microbiology and Biotechnology, College of Natural Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Hakim SendagireDepartment of Microbiology, Makerere University, College of Health Sciences, P. O. Box 7062, Kampala, Uganda.
Makerere University · UGIslamic University in Uganda · UG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHepatitis B virus (HBV) is the leading cause of liver-related diseases. In Uganda, there is a regional disparity in the HBV burden. Our study was aimed at establishing the circulating genotypes in a low and a high endemic region to give plausible explanations for the differences in regional burden and guide the future management of the disease.

methodsA total of 200 HBsAg-seropositive subjects were recruited into the study by convenience sampling. The HBsAg Rapid Test Strip (Healgen Scientific Limited Liability Company, Houston, TX77047- USA) was used to screen for HBsAg while the Roche machine (Roche, Basel Switzerland/Abbot Technologies (USA)) was used to determine the viral load. The Chemistry Analyzer B120 (Mindray, China) was used for chemistry analysis. For HBV genotyping, total DNA was extracted from whole blood using the QIAamp® DNA extraction kit. Nested PCR amplification was performed using Platinum Taq DNA Polymerase (Invitrogen Corporation, USA) to amplify the 400 bp HBV polymerase gene. Purification of nested PCR products was performed using Purelink PCR product purification kit (Life Technologies, USA). Automated DNA sequencing was performed using BigDye Terminator v3.1 Cycle Sequencing Kit on 3130 Genetic Analyzer (Applied Biosystems, USA). The NCBI HBV genotyping tool (https://www.ncbi.nlm.nih.gov/projects/genotyping/formpage.cgi) was used for determination of genotype for each HBV sequence. Pearson's chi-square, multinomial logistic regression, and Mann-Whitney

resultsMajority of our study subjects were female (64.5%), youth (51.0%), and married (62.0%). Overall, genotype A was the most prevalent (46%). Genotype D and the recombinant genotype D/E were proportionately more distributed in the high endemic (38.2%) and low endemic (36.5%) regions, respectively. Genotype D was significantly more prevalent in the high endemic region and among the elderly (

conclusionThere is disproportionate distribution of genotypes A and D and the recombinant genotype D/E in the low and high endemic regions of Uganda. This probably could explain the differences in endemicity of HBV in our country signifying the need for regional specific HBV management and control strategies.

Identifiers

PMID35070455
PMCPMC8767397
OpenAlexW4206001676

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.