Evidence map›Paper›PMID 35070229›Full record

ArticleJournal of healthcare engineering2022

GP96 and SMP30 Protein Priming of Dendritic Cell Vaccination Induces a More Potent CTL Response against Hepatoma.

Rongshi Huang, Jian Pan, Yaoyao Zhang, Qiuhong Qin, Naixia Chao, Tianming Huang, Chengxiao Chen, Feilan Zhao, Guorong Luo

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of healthcare engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Rongshi HuangDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Medical University, Nanning,530021, China.
Jian PanDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Medical University, Nanning,530021, China.
Yaoyao ZhangDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Medical University, Nanning,530021, China.
Qiuhong QinDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Medical University, Nanning,530021, China.
Naixia ChaoDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Medical University, Nanning,530021, China.
Tianming HuangDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Medical University, Nanning,530021, China.
Chengxiao ChenDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Medical University, Nanning,530021, China.
Feilan ZhaoDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Traditional Chinese Medical University, Nanning,530001, China.ORCID 0000-0002-1599-6627
Guorong LuoDepartment of Histology and Embryology, Institute of Preclinical,Guangxi Medical University, Nanning,530021, China.ORCID 0000-0002-1484-0364
Guangxi Medical University · CNGuangxi University of Chinese Medicine · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heat-shock protein (HSP) GP96 is a well-known adjuvant in immunotherapy. It belongs to the HSP90 family. Our previous study demonstrated that DC pulsed with recombinant senescence marker protein 30 (SMP30) could induce cytotoxic T lymphocytes (CTLs) against liver cancer cells in vitro. In this study, SMP30 and GP96 were subcloned into lentiviruses and transfected into DCs from healthy donors. We included six groups: the GP96-SMP30 group, GP96 group, SMP30 group, DC group, empty vector control group, and hepatoma extracted protein group. We used ELISA to detect cytokines and flow cytometry to assess CD80 and CD86 on DCs and the effect of CTLs. Our vector design was considered successful and further studied. In the SMP30 group, DC expresses more CCR7 and CD86 than the control group; in the SMP30+GP96 group, DC express more CCR7, CD86, and CD80 than the control group. Transfected DCs secreted more TNF-

Indexed as

Carcinoma, HepatocellularLiver NeoplasmsCytokinesDendritic CellsHumansReceptors, CCR7T-Lymphocytes, CytotoxicTumor Necrosis Factor-alphaVaccinationCytokinesReceptors, CCR7Tumor Necrosis Factor-alpha

Identifiers

PMID35070229
PMCPMC8767371
OpenAlexW4206782443

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.