ArticleFrontiers in microbiology2021
Real-Time Monitoring of the Yeast Intracellular State During Bioprocesses With a Toolbox of Biosensors.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Do seconds make a difference? Investigating strain-specific behavior of yeast in dynamic glucose environments.Microbial cell factories · 2026Article
- The return of metabolism: biochemistry and physiology of glycolysis.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Single cell profiling framework reveals metabolic subpopulations as drivers of bioproduction heterogeneity.Nature communications · 2025Article
- Physiology and Robustness of Yeasts Exposed to Dynamic pH and Glucose Environments.Biotechnology and bioengineering · 2025Article
- Expanding the Yeast MoClo Toolkit: gene expression control parts forSynthetic biology (Oxford, England) · 2025Article
- Robustness quantification of a mutant library screen revealed key genetic markers in yeast.Microbial cell factories · 2024Article
- Evaluation of Pyrophosphate-Driven Proton Pumps inMicroorganisms · 2024Article
- Engineering Saccharomyces cerevisiae for targeted hydrolysis and fermentation of glucuronoxylan through CRISPR/Cas9 genome editing.Microbial cell factories · 2024Article
- POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology.ACS synthetic biology · 2024Article
- Quantifying microbial robustness in dynamic environments using microfluidic single-cell cultivation.Microbial cell factories · 2024Article
- A transcriptomic atlas of acute stress response to low pH in multipleMicrobiology spectrum · 2024Article
- Automated yeast cultivation control using a biosensor and flow cytometry.Journal of industrial microbiology & biotechnology · 2024Article
- Performance and robustness analysis reveals phenotypic trade-offs in yeast.Life science alliance · 2024Article
- Four ways of implementing robustness quantification in strain characterisation.Biotechnology for biofuels and bioproducts · 2023Article
- Article
- Physiology of Saccharomyces cerevisiae during growth on industrial sugar cane molasses can be reproduced in a tailor-made defined synthetic medium.Scientific reports · 2023Article
- Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells.Journal of visualized experiments : JoVE · 2023Article
- Assessment of the TRX2p-yEGFP Biosensor to Monitor the Redox Response of an Industrial Xylose-FermentingJournal of fungi (Basel, Switzerland) · 2023Article
- Real-Time Assessment of Intracellular Metabolites in Single Cells through RNA-Based Sensors.Biomolecules · 2023Review
- Confocal imaging of biomarkers at a single-cell resolution: quantifying 'living' in 3D-printable engineered living material based on Pluronic F-127 and yeast Saccharomyces cerevisiae.Biomaterials research · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Industrial fermentation processes strive for high robustness to ensure optimal and consistent performance. Medium components, fermentation products, and physical perturbations may cause stress and lower performance. Cellular stress elicits a range of responses, whose extracellular manifestations have been extensively studied; whereas intracellular aspects remain poorly known due to lack of tools for real-time monitoring. Genetically encoded biosensors have emerged as promising tools and have been used to improve microbial productivity and tolerance toward industrially relevant stresses. Here, fluorescent biosensors able to sense the yeast intracellular environment (pH, ATP levels, oxidative stress, glycolytic flux, and ribosome production) were implemented into a versatile and easy-to-use toolbox. Marker-free and efficient genome integration at a conserved site on chromosome X of Saccharomyces cerevisiae strains and a commercial Saccharomyces boulardii strain was developed. Moreover, multiple biosensors were used to simultaneously monitor different intracellular parameters in a single cell. Even when combined together, the biosensors did not significantly affect key physiological parameters, such as specific growth rate and product yields. Activation and response of each biosensor and their interconnection were assessed using an advanced micro-cultivation system. Finally, the toolbox was used to screen cell behavior in a synthetic lignocellulosic hydrolysate that mimicked harsh industrial substrates, revealing differences in the oxidative stress response between laboratory (CEN.PK113-7D) and industrial (Ethanol Red) S. cerevisiae strains. In summary, the toolbox will allow both the exploration of yeast diversity and physiological responses in natural and complex industrial conditions, as well as the possibility to monitor production processes.
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