Evidence map›Paper›PMID 35069110›Full record

ArticleFrontiers in molecular neuroscience2021

A Versatile Clustered Regularly Interspaced Palindromic Repeats Toolbox to Study Neurological Ca

Despina Tsortouktzidis, Anna R Tröscher, Herbert Schulz, Thoralf Opitz, Susanne Schoch, Albert J Becker, Karen M J van Loo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in molecular neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 100% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Despina TsortouktzidisInstitute of Neuropathology, Medical Faculty, Section for Translational Epilepsy Research, University of Bonn, Bonn, Germany.
Anna R TröscherInstitute of Neuropathology, Medical Faculty, Section for Translational Epilepsy Research, University of Bonn, Bonn, Germany.
Herbert SchulzDepartment of Microgravity and Translational Regenerative Medicine, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, Magdeburg, Germany.
Thoralf OpitzInstitute of Experimental Epileptology and Cognition Research, Medical Faculty, University of Bonn, Bonn, Germany.
Susanne SchochInstitute of Neuropathology, Medical Faculty, Section for Translational Epilepsy Research, University of Bonn, Bonn, Germany.
Albert J BeckerInstitute of Neuropathology, Medical Faculty, Section for Translational Epilepsy Research, University of Bonn, Bonn, Germany.
Karen M J van LooInstitute of Neuropathology, Medical Faculty, Section for Translational Epilepsy Research, University of Bonn, Bonn, Germany.
University of Bonn · DEJohannes Kepler University of Linz · ATOtto-von-Guericke University Magdeburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Precise genome editing in combination with viral delivery systems provides a valuable tool for neuroscience research. Traditionally, the role of genes in neuronal circuits has been addressed by overexpression or knock-out/knock-down systems. However, those techniques do not manipulate the endogenous loci and therefore have limitations. Those constraints include that many genes exhibit extensive alternative splicing, which can be regulated by neuronal activity. This complexity cannot be easily reproduced by overexpression of one protein variant. The CRISPR activation and interference/inhibition systems (CRISPRa/i) directed to promoter sequences can modulate the expression of selected target genes in a highly specific manner. This strategy could be particularly useful for the overexpression of large proteins and for alternatively spliced genes, e.g., for studying large ion channels known to be affected in ion channelopathies in a variety of neurological diseases. Here, we demonstrate the feasibility of a newly developed CRISPRa/i toolbox to manipulate the promoter activity of the

Indexed as

Cacna1h promoter modulationCRISPR-induced activationCRISPR-induced inhibitionion channelopathiesT-Type calcium channel Cav3.2

Identifiers

PMID35069110
PMCPMC8770422
OpenAlexW4205591370

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.