Evidence map›Paper›PMID 35067494›Full record

ArticleThe Journal of veterinary medical science2022

Semen collection by urethral catheterization and electro-ejaculation with different voltages, and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (Macaca fuscata).

Noboru Takaesu, Chihiro Kanno, Kosuke Sugimoto, Masashi Nagano, Akihisa Kaneko, Yoriko Indo, Hiroo Imai, Hirohisa Hirai, Munehiro Okamoto, Mariko Sashika and 4 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. A Descriptive Study of Brown Bear (Animals : an open access journal from MDPI · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Noboru TakaesuLaboratory of Wildlife Biology and Medicine, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Chihiro KannoLaboratory of Clinical Veterinary Medicine for Large Animal, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Kosuke SugimotoLaboratory of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Masashi NaganoLaboratory of Animal Reproduction, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
Akihisa KanekoPrimate Research Institute, Kyoto University, Aichi, Japan.
Yoriko IndoPrimate Research Institute, Kyoto University, Aichi, Japan.
Hiroo ImaiPrimate Research Institute, Kyoto University, Aichi, Japan.
Hirohisa HiraiPrimate Research Institute, Kyoto University, Aichi, Japan.
Munehiro OkamotoPrimate Research Institute, Kyoto University, Aichi, Japan.
Mariko SashikaLaboratory of Wildlife Biology and Medicine, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Michito ShimozuruLaboratory of Wildlife Biology and Medicine, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Seiji KatagiriLaboratory of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Toshio TsubotaLaboratory of Wildlife Biology and Medicine, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Yojiro YanagawaLaboratory of Theriogenology, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Hokkaido University · JPKyoto University · JPKitasato University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the Japanese macaque, semen has been collected by electro-ejaculation (EE), using the higher voltage stimuli compared to other species including genus Macaca. Semen coagulates immediately after ejaculation, which makes difficult to produce high-quality semen for artificial insemination. Recently, semen collection using urethral catheterization (UC) has been reported in carnivore and this technique may allow semen collection without coagulation in a less invasive manner. Further, the temporal preservation temperature and cooling rate of semen during cryopreservation affect post thawing sperm quality. In this study, to improve semen quality and quantity, as well as the animal welfare, semen collection was performed by EE with high (5-15 V) or low (3-6 V) voltage, UC and a combination of the two (EE-UC). It has been suggested that a high voltage is necessary for semen collection, but 10 V stimulation was effective enough and 15 V is for additional sperm collection. Also, liquid semen was collected by EE-UC and this could increase the total number of sperm. Further, to improve the post thawing sperm motility, semen was kept at four temperatures (4, 15, 25 and 37°C) for 60 min, and processed with two cooling procedures (slow cooling before second dilution and fast cooling after second dilution). Holding semen at 25°C and fast cooling after the second dilution maintained progressive motile sperm rate. The present results will contribute to the improvement of semen collection and animal welfare of Japanese macaques.

Indexed as

Semen AnalysisSemen PreservationAnimalsCryopreservationEjaculationMacacaMacaca fuscataMaleSemenSpermatozoaSperm MotilityTemperatureUrinary Catheterizationcryopreservationelectro-ejaculationJapanese macaquessemen collectionurethral catheterization

Identifiers

PMID35067494
PMCPMC8983288
OpenAlexW4207082873

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.