Evidence map›Paper›PMID 35066770›Full record

ArticleMolecular biology reports2022

Neutrophil extracellular traps (NETs) modulate inflammatory profile in obese humans and mice: adipose tissue role on NETs levels.

Daniela Fernanda Freitas, David Fernando Colón, Rangel Leal Silva, Eloá Mangabeira Santos, Victor Hugo Dantas Guimarães, Guilherme Henrique Mendes Ribeiro, Alfredo Maurício Batista de Paula, André Luiz Sena Guimarães, Sidnei Tavares Dos Reis, Fernando Queiroz Cunha and 3 more

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

  1. Systematic review: The gut microbiota as a link between colorectal cancer and obesity.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Daniela Fernanda FreitasLaboratory of Health Science, Postgraduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil.
David Fernando ColónCenter for Research in Inflammatory Diseases (CRID), Department of Pharmacology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, Brazil.
Rangel Leal SilvaCenter for Research in Inflammatory Diseases (CRID), Department of Pharmacology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, Brazil.
Eloá Mangabeira SantosLaboratory of Health Science, Postgraduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil.
Victor Hugo Dantas GuimarãesLaboratory of Health Science, Postgraduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil.
Guilherme Henrique Mendes RibeiroInstitute of Agriculture Sciences, Departments of Food Engineering, Federal University of Minas Gerais (UFMG), Bairro Universitário, Montes Claros, Minas Gerais, Brazil.
Alfredo Maurício Batista de PaulaLaboratory of Health Science, Postgraduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil.
André Luiz Sena GuimarãesLaboratory of Health Science, Postgraduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil.
Sidnei Tavares Dos ReisInstitute of Agriculture Sciences, Departments of Food Engineering, Federal University of Minas Gerais (UFMG), Bairro Universitário, Montes Claros, Minas Gerais, Brazil.
Fernando Queiroz CunhaCenter for Research in Inflammatory Diseases (CRID), Department of Pharmacology, Ribeirão Preto Medical School, University of Sao Paulo, Ribeirão Preto, Brazil.
Maisa Mota AntunesCenter for Gastrointestinal Biology, Departments of Morphology, Federal University of Minas Gerais, Minas Gerais, Brazil.
Gustavo Batista MenezesCenter for Gastrointestinal Biology, Departments of Morphology, Federal University of Minas Gerais, Minas Gerais, Brazil.
Sérgio Henrique Sousa SantosLaboratory of Health Science, Postgraduate Program in Health Sciences, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil. sergiosousas@hotmail.com.ORCID http://orcid.org/0000-0002-7788-5447
Universidade Estadual de Montes Claros · BRUniversidade Federal de Minas Gerais · BRUniversidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNeutrophil extracellular traps (NETs) are a recently discovered neutrophil defense mechanism which modulates several inflammatory conditions contributing to metabolic profile alterations. Therefore, the present study aimed to evaluate the production of NETs in obese patients and mice, verifying the possible mechanisms associated with the release of NETs by the adipose tissue. METHODS AND

resultsThe present study investigated NETs production in human adipose tissue and also showing the neutrophils using intravital microscopy in mouse epididymal adipose tissue. Blood and white adipose tissues were obtained from eutrophic and obese individuals and from mice. Lipid, glycemic and leukocyte profiles were evaluated, as well as the levels of NETs and its markers. Bioinformatics and proteomics analyses were performed and the identified key proteins were measured. The main findings showed that the inflammatory markers interleukin-8 (IL-8), heat shock protein 90 (HSP90) and the E1 heat shock protein family (HSPE1) can be modulated by the NETs levels in obesity. Obesity has also been associated with increased cholesterol, glucose intolerance, ionic calcium and NETs. We also observed an increase in catalase and a decreased superoxide dismutase activity. Bioinformatics and proteomics analyses revealed that IL-8, HSP90 and HSPE1 were associated with obesity, inflammation and NETs release.

conclusionsIn conclusion, the present study shows an increase in NETs production during obesity associated with important inflammatory markers in adipose.

Indexed as

Extracellular TrapsAdipose TissueAnimalsHumansInflammationMiceNeutrophilsObesityAdiposeInflammationNeutrophil extracellular trapsObesityProteomicsTranslational study

Identifiers

PMID35066770
OpenAlexW4207019870

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.