Evidence map›Paper›PMID 35066669›Full record

ArticleJournal of bone and mineral metabolism2022

LncRNA XIST facilitates S1P-mediated osteoclast differentiation via interacting with FUS.

Da-Wei Zhang, Hong-Gang Wang, Kui-Bo Zhang, Yuan-Qing Guo, Lian-Jun Yang, Hai Lv

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of bone and mineral metabolism, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
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  6. Role and Regulation of Transcription Factors in Osteoclastogenesis.International journal of molecular sciences · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Da-Wei ZhangDepartment of Spinal Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, No. 52, Meihua East Road, Xiangzhou District, Zhuhai, 519000, Guangdong Province, China.
Hong-Gang WangDepartment of Orthopaedic and Microsurgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, Guangdong Province, China.
Kui-Bo ZhangDepartment of Spinal Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, No. 52, Meihua East Road, Xiangzhou District, Zhuhai, 519000, Guangdong Province, China.
Yuan-Qing GuoDepartment of Spinal Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, No. 52, Meihua East Road, Xiangzhou District, Zhuhai, 519000, Guangdong Province, China.
Lian-Jun YangDepartment of Spinal Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, No. 52, Meihua East Road, Xiangzhou District, Zhuhai, 519000, Guangdong Province, China.
Hai LvDepartment of Spinal Surgery, The Fifth Affiliated Hospital of Sun Yat-Sen University, No. 52, Meihua East Road, Xiangzhou District, Zhuhai, 519000, Guangdong Province, China. lvhai@mail.sysu.edu.cn.ORCID http://orcid.org/0000-0001-5039-3939
Sun Yat-sen University · CN

Funding

Guangdong Natural Science Foundation Committee, Doctoral Startup Fund 2018a03010282
6 · The paper itself

Abstract

introductionThe diagnosis and treatment of osteoporosis, a frequent age-related metabolic bone disorder, remain incomprehensive and challenging. The potential regulatory role of lncRNA XIST and sphingosine kinase 1 (SPHK1) pathway need experimental investigations. MATERIALS AND

methodsRAW264.7 cells and BMMs were obtained for in vitro studies and 30 ng/mL RANKL was implemented for induction of osteoclast differentiation. The suppressing of lncRNA XIST, SPHK1 and fused in sarcoma (FUS) was achieved using small hairpin RNA, while overexpression of XIST and FUS was constructed by pcDNA3.1 vector system. Tartrate-resistant acid phosphatase (TRAP) staining was used for observation of formation of osteoclasts. RNA-pulldown analysis and RNA binding protein immunoprecipitation (RIP) was implemented for measuring mRNA and protein interactions. RT-qPCR was conducted to determining mRNA expression, whereas ELISA and Western blotting assay was performed for monitoring protein expression.

resultsRANKL induced osteoclast differentiation and upregulated expression of osteoclastogenesis-related genes that included NFATc1, CTSK, TRAP and SPHK1 and the level of lncRNA XIST in both RAW264.7 cells and BMMs. However, knockdown of lncRNA XIST or suppressing SPHK1 significantly reserved the effects of RANKL. LncRNA XIST was further demonstrated to be interacted with FUS and increased the stability of SPHK1, indicating its ability in promoting osteoclast differentiation through SPHK1/S1P/ERK signaling pathway.

conclusionLncRNA XIST promoted osteoclast differentiation via interacting with FUS and upregulating SPHK1/S1P/ERK pathway.

Indexed as

Bone ResorptionOsteoclastsRNA, Long NoncodingAnimalsCathepsin KCell DifferentiationHematopoiesisMiceNFATC Transcription FactorsOsteogenesisPhosphotransferases (Alcohol Group Acceptor)Proprotein ConvertasesRANK LigandRAW 264.7 CellsRNA-Binding Protein FUSSerine EndopeptidasesAcp5 protein, mouseCathepsin KCtsk protein, mouseFUS protein, mousemembrane-bound transcription factor peptidase, site 1NFATC Transcription FactorsPhosphotransferases (Alcohol Group Acceptor)Proprotein ConvertasesRANK LigandRNA-Binding Protein FUSRNA, Long NoncodingSerine EndopeptidasesSphingosine KinaseSphk1 protein, mouseTartrate-Resistant Acid PhosphataseXIST non-coding RNAFUSLncRNA XISTOsteoclast differentiationSPHK1

Identifiers

PMID35066669
OpenAlexW4207065873

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.