ArticleNucleic acids research2022
A phage parasite deploys a nicking nuclease effector to inhibit viral host replication.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 47 citations in OpenAlex.
- Repurposing anti-phage defenses to differentially arrest the viral lifecycle reveals the regulatory logic of a parasitic satellite.mSystems · 2026Article
- Shuttling, swapping, and mixing: the rapid modular evolution of antiviral repertoires in temperate phages and their satellites.Nucleic acids research · 2026Article
- Ec_Ou_ZC1, a new Tunavirus, reveals Cor superinfection exclusion and differential Avs susceptibility across Drexlerviridae subfamilies.BMC microbiology · 2026Article
- Article
- Repurposing anti-phage defenses to differentially arrest the viral lifecycle reveals the regulatory logic of a parasitic satellite.bioRxiv : the preprint server for biology · 2026Article
- Article
- Diversity and evolution of archaeal immune strategies.Nucleic acids research · 2026Article
- Rapid CRISPR-Cas9 target-strand nicking can provide phage resistance by reducing DNA abundance.Nucleic acids research · 2025Article
- Why do bacteria accumulate antiphage defence systems?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Lineage-specific defence systems of pandemicPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- A DNA nicking Class 1 OLD family nuclease mediates phage defense in Vibrio cholerae and is countered by a phage-encoded inhibitor.Nucleic acids research · 2025Article
- Genetics, ecology and evolution of phage satellites.Nature reviews. Microbiology · 2025Review
- Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains.Molecular cell · 2025Article
- Tandem mobilization of anti-phage defenses alongside SCCmec elements in staphylococci.Nature communications · 2024Article
- A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread.Nucleic acids research · 2024Article
- Nucleoid-associated proteins shape the global protein occupancy and transcriptional landscape of a clinical isolate ofmSphere · 2024Article
- A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread.bioRxiv : the preprint server for biology · 2024Article
- AeLife · 2024Article
- AbioRxiv : the preprint server for biology · 2023Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.NAR genomics and bioinformatics · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
PLEs (phage-inducible chromosomal island-like elements) are phage parasites integrated into the chromosome of epidemic Vibrio cholerae. In response to infection by its viral host ICP1, PLE excises, replicates and hijacks ICP1 structural components for transduction. Through an unknown mechanism, PLE prevents ICP1 from transitioning to rolling circle replication (RCR), a prerequisite for efficient packaging of the viral genome. Here, we characterize a PLE-encoded nuclease, NixI, that blocks phage development likely by nicking ICP1's genome as it transitions to RCR. NixI-dependent cleavage sites appear in ICP1's genome during infection of PLE(+) V. cholerae. Purified NixI demonstrates in vitro nuclease activity specifically for sites in ICP1's genome and we identify a motif that is necessary for NixI-mediated cleavage. Importantly, NixI is sufficient to limit ICP1 genome replication and eliminate progeny production, representing the most inhibitory PLE-encoded mechanism revealed to date. We identify distant NixI homologs in an expanded family of putative phage parasites in vibrios that lack nucleotide homology to PLEs but nonetheless share genomic synteny with PLEs. More generally, our results reveal a previously unknown mechanism deployed by phage parasites to limit packaging of their viral hosts' genome and highlight the prominent role of nuclease effectors as weapons in the arms race between antagonizing genomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.